Objectives: a new hydrophilic sealer based on calcium-fluoro-silicate cement was tested for its sealing, biocompatibility and bioactivity (apatite forming ability) properties. Methods: extracted single-rooted teeth were instrumented with ProTaper rotary instruments, irrigated with NaOCl, EDTA, and filled with single-cone gutta-percha (Hygienic,USA) in association with TECHBiosealer Endo (Isasan,Italy) (N=10) or CRCS (Hygienic,USA) (N=10), and measured for apical flow at 24 hours and 1,4 weeks aging in DPBS (Dulbecco's Phosphate Buffered Saline) at 37°C by a digital fluid flow-meter (University of Bologna,Italy). Apatite formation was evaluated by ESEM/EDX, Raman and FTIR analyses on materials as-prepared or aged for 6,24 hours and 1,4 weeks in DPBS. Osteoblast-like Saos-2 cells seeded on cement were tested for viability at 1,3,7 days. Results: statistical differences in fluid flow (p<0.05) among the sealers were found at all analysis time-endpoints. CRCS group showed higher apical microleakage than TECHBiosealer. ESEM of fresh TECHBiosealer showed the formation of a dense gel and many crystals on the surface after 6 hours. EDX revealed high amounts of calcium and silicon on fresh, 6 and 24 hours-aged samples, while Ca and P were found on 1 week and 1 month-aged samples. Diffuse deposits of apatite spherulites were detected on 1 and 4 weeks-aged TECHBiosealer samples. Raman and FTIR analyses showed C-S-H and calcium-hydroxide bands after 6 hours, whereas apatite bands were observed after 24 hours. No bioactivity was demonstrated by CRCS. Viability of Saos-2 cells on TECHBiosealer was higher than on CRCS (Table: Alamar blue assay, n=3, relative fluorescence units). Conclusions: TECHBiosealer Endo has the capacity to form apatite and an apical sealing ability better than CRCS. Early calcium-hydroxide and C-S-H gel formation promote the nucleation of apatite deposits, that play a positive role in inducing cell growth and root-canal sealing. The formation of apatite may improve the apical sealing and biocompatibility.
Biocompatibility and sealing of TECHBiosealer Endo as function of bioactivity
GANDOLFI, MARIA GIOVANNA;SIBONI, FRANCESCO;TADDEI, PAOLA;PRATI, CARLO
2010
Abstract
Objectives: a new hydrophilic sealer based on calcium-fluoro-silicate cement was tested for its sealing, biocompatibility and bioactivity (apatite forming ability) properties. Methods: extracted single-rooted teeth were instrumented with ProTaper rotary instruments, irrigated with NaOCl, EDTA, and filled with single-cone gutta-percha (Hygienic,USA) in association with TECHBiosealer Endo (Isasan,Italy) (N=10) or CRCS (Hygienic,USA) (N=10), and measured for apical flow at 24 hours and 1,4 weeks aging in DPBS (Dulbecco's Phosphate Buffered Saline) at 37°C by a digital fluid flow-meter (University of Bologna,Italy). Apatite formation was evaluated by ESEM/EDX, Raman and FTIR analyses on materials as-prepared or aged for 6,24 hours and 1,4 weeks in DPBS. Osteoblast-like Saos-2 cells seeded on cement were tested for viability at 1,3,7 days. Results: statistical differences in fluid flow (p<0.05) among the sealers were found at all analysis time-endpoints. CRCS group showed higher apical microleakage than TECHBiosealer. ESEM of fresh TECHBiosealer showed the formation of a dense gel and many crystals on the surface after 6 hours. EDX revealed high amounts of calcium and silicon on fresh, 6 and 24 hours-aged samples, while Ca and P were found on 1 week and 1 month-aged samples. Diffuse deposits of apatite spherulites were detected on 1 and 4 weeks-aged TECHBiosealer samples. Raman and FTIR analyses showed C-S-H and calcium-hydroxide bands after 6 hours, whereas apatite bands were observed after 24 hours. No bioactivity was demonstrated by CRCS. Viability of Saos-2 cells on TECHBiosealer was higher than on CRCS (Table: Alamar blue assay, n=3, relative fluorescence units). Conclusions: TECHBiosealer Endo has the capacity to form apatite and an apical sealing ability better than CRCS. Early calcium-hydroxide and C-S-H gel formation promote the nucleation of apatite deposits, that play a positive role in inducing cell growth and root-canal sealing. The formation of apatite may improve the apical sealing and biocompatibility.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
