Objectives: a new hydrophilic sealer based on calcium-fluoro-silicate cement was tested for its sealing, biocompatibility and bioactivity (apatite forming ability) properties. Methods: extracted single-rooted teeth were instrumented with ProTaper rotary instruments, irrigated with NaOCl, EDTA, and filled with single-cone gutta-percha (Hygienic,USA) in association with TECHBiosealer Endo (Isasan,Italy) (N=10) or CRCS (Hygienic,USA) (N=10), and measured for apical flow at 24 hours and 1,4 weeks aging in DPBS (Dulbecco's Phosphate Buffered Saline) at 37°C by a digital fluid flow-meter (University of Bologna,Italy). Apatite formation was evaluated by ESEM/EDX, Raman and FTIR analyses on materials as-prepared or aged for 6,24 hours and 1,4 weeks in DPBS. Osteoblast-like Saos-2 cells seeded on cement were tested for viability at 1,3,7 days. Results: statistical differences in fluid flow (p<0.05) among the sealers were found at all analysis time-endpoints. CRCS group showed higher apical microleakage than TECHBiosealer. ESEM of fresh TECHBiosealer showed the formation of a dense gel and many crystals on the surface after 6 hours. EDX revealed high amounts of calcium and silicon on fresh, 6 and 24 hours-aged samples, while Ca and P were found on 1 week and 1 month-aged samples. Diffuse deposits of apatite spherulites were detected on 1 and 4 weeks-aged TECHBiosealer samples. Raman and FTIR analyses showed C-S-H and calcium-hydroxide bands after 6 hours, whereas apatite bands were observed after 24 hours. No bioactivity was demonstrated by CRCS. Viability of Saos-2 cells on TECHBiosealer was higher than on CRCS (Table: Alamar blue assay, n=3, relative fluorescence units). Conclusions: TECHBiosealer Endo has the capacity to form apatite and an apical sealing ability better than CRCS. Early calcium-hydroxide and C-S-H gel formation promote the nucleation of apatite deposits, that play a positive role in inducing cell growth and root-canal sealing. The formation of apatite may improve the apical sealing and biocompatibility.
M.G. Gandolfi, F. Siboni, G. Piras, S. Pisu, P. Taddei, G. Ciapetti, et al. (2010). Biocompatibility and sealing of TECHBiosealer Endo as function of bioactivity.
Biocompatibility and sealing of TECHBiosealer Endo as function of bioactivity
GANDOLFI, MARIA GIOVANNA;SIBONI, FRANCESCO;TADDEI, PAOLA;PRATI, CARLO
2010
Abstract
Objectives: a new hydrophilic sealer based on calcium-fluoro-silicate cement was tested for its sealing, biocompatibility and bioactivity (apatite forming ability) properties. Methods: extracted single-rooted teeth were instrumented with ProTaper rotary instruments, irrigated with NaOCl, EDTA, and filled with single-cone gutta-percha (Hygienic,USA) in association with TECHBiosealer Endo (Isasan,Italy) (N=10) or CRCS (Hygienic,USA) (N=10), and measured for apical flow at 24 hours and 1,4 weeks aging in DPBS (Dulbecco's Phosphate Buffered Saline) at 37°C by a digital fluid flow-meter (University of Bologna,Italy). Apatite formation was evaluated by ESEM/EDX, Raman and FTIR analyses on materials as-prepared or aged for 6,24 hours and 1,4 weeks in DPBS. Osteoblast-like Saos-2 cells seeded on cement were tested for viability at 1,3,7 days. Results: statistical differences in fluid flow (p<0.05) among the sealers were found at all analysis time-endpoints. CRCS group showed higher apical microleakage than TECHBiosealer. ESEM of fresh TECHBiosealer showed the formation of a dense gel and many crystals on the surface after 6 hours. EDX revealed high amounts of calcium and silicon on fresh, 6 and 24 hours-aged samples, while Ca and P were found on 1 week and 1 month-aged samples. Diffuse deposits of apatite spherulites were detected on 1 and 4 weeks-aged TECHBiosealer samples. Raman and FTIR analyses showed C-S-H and calcium-hydroxide bands after 6 hours, whereas apatite bands were observed after 24 hours. No bioactivity was demonstrated by CRCS. Viability of Saos-2 cells on TECHBiosealer was higher than on CRCS (Table: Alamar blue assay, n=3, relative fluorescence units). Conclusions: TECHBiosealer Endo has the capacity to form apatite and an apical sealing ability better than CRCS. Early calcium-hydroxide and C-S-H gel formation promote the nucleation of apatite deposits, that play a positive role in inducing cell growth and root-canal sealing. The formation of apatite may improve the apical sealing and biocompatibility.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
