Objectives: sealing ability and apatite forming ability (bioactivity) of a new endodontic sealer based on calcium-fluoro-silicate hydraulic cement were tested. Methods: extracted single-rooted teeth were instrumented with ProTaper rotary instruments, irrigated with NaOCl, EDTA, and filled with single-cone gutta-percha (Hygienic,USA) in association with TECHBiosealer Endo (Isasan,Italy) (N=10) or CRCS (Hygienic,USA) (N=10). The apical leakage was measured after 24 hours, 1,4 weeks and 3 months of aging in simulated body fluid (Hanks' Balanced Salt Solution, HBSS) at 37°C by a digital fluid flow-meter (University of Bologna, Italy). The bioactivity i.e. apatite formation ability was assessed by ESEM/EDX, micro-Raman and FTIR analyses on materials as-prepared or aged for 6,24 hours and 1,4 weeks in HBSS. Results: TECHBiosealer group showed statistically lower apical microleakage than CRCS group. Statistical differences in fluid flow (p<0.05) among the sealers were found at all analysis time-endpoints. The seal of TECHBiosealer endo was stable over time, showing the following apical leakage (µL/min): 0.043 ± 0.038 at 24 hours, 0.025 ± 0.020 at 1 week, 0.040 ± 0.015 at 4 weeks and 0.079 ± 0.035 at 3 months. Differently fluid flow of CRCS increase over time (µL/min): 0.106 ± 0.117 at 24 hours, 0.139 ± 0.176 at 1 week, 0.128 ± 0.105 at 4 weeks and 0.232 ± 0.227 at 3 months. ESEM of fresh TECHBiosealer showed the formation of a dense gel and many crystals on the surface after 6 hours in HBSS. EDX revealed high amounts of calcium and silicon on fresh, 6 and 24 hours-aged samples, while Ca and P were found on 1 week and on 1 month-aged samples. Diffuse deposits of apatite spherulites were detected on 1 and 4 weeks-aged TECHBiosealer samples. Micro-Raman and FTIR analyses showed C-S-H and calcium-hydroxide bands after 6 hours, whereas apatite bands were observed after 24 hours in HBSS. No bioactivity was demonstrated by CRCS. Conclusions: TECHBiosealer Endo has the capacity to form apatite and possess an apical sealing ability better than CRCS. Early calcium-hydroxide and C-S-H gel formation promote the nucleation of apatite deposits. The formation of apatite improve the apical sealing.
M.G. Gandolfi, F. Siboni, G. Piras, S. Pisu, A. Colin, P. Taddei, et al. (2010). Apatite forming ability (bioactivity) of TECHBiosealer Endo improves the sealing ability. s.l : s.n.
Apatite forming ability (bioactivity) of TECHBiosealer Endo improves the sealing ability
GANDOLFI, MARIA GIOVANNA;SIBONI, FRANCESCO;TADDEI, PAOLA;PRATI, CARLO
2010
Abstract
Objectives: sealing ability and apatite forming ability (bioactivity) of a new endodontic sealer based on calcium-fluoro-silicate hydraulic cement were tested. Methods: extracted single-rooted teeth were instrumented with ProTaper rotary instruments, irrigated with NaOCl, EDTA, and filled with single-cone gutta-percha (Hygienic,USA) in association with TECHBiosealer Endo (Isasan,Italy) (N=10) or CRCS (Hygienic,USA) (N=10). The apical leakage was measured after 24 hours, 1,4 weeks and 3 months of aging in simulated body fluid (Hanks' Balanced Salt Solution, HBSS) at 37°C by a digital fluid flow-meter (University of Bologna, Italy). The bioactivity i.e. apatite formation ability was assessed by ESEM/EDX, micro-Raman and FTIR analyses on materials as-prepared or aged for 6,24 hours and 1,4 weeks in HBSS. Results: TECHBiosealer group showed statistically lower apical microleakage than CRCS group. Statistical differences in fluid flow (p<0.05) among the sealers were found at all analysis time-endpoints. The seal of TECHBiosealer endo was stable over time, showing the following apical leakage (µL/min): 0.043 ± 0.038 at 24 hours, 0.025 ± 0.020 at 1 week, 0.040 ± 0.015 at 4 weeks and 0.079 ± 0.035 at 3 months. Differently fluid flow of CRCS increase over time (µL/min): 0.106 ± 0.117 at 24 hours, 0.139 ± 0.176 at 1 week, 0.128 ± 0.105 at 4 weeks and 0.232 ± 0.227 at 3 months. ESEM of fresh TECHBiosealer showed the formation of a dense gel and many crystals on the surface after 6 hours in HBSS. EDX revealed high amounts of calcium and silicon on fresh, 6 and 24 hours-aged samples, while Ca and P were found on 1 week and on 1 month-aged samples. Diffuse deposits of apatite spherulites were detected on 1 and 4 weeks-aged TECHBiosealer samples. Micro-Raman and FTIR analyses showed C-S-H and calcium-hydroxide bands after 6 hours, whereas apatite bands were observed after 24 hours in HBSS. No bioactivity was demonstrated by CRCS. Conclusions: TECHBiosealer Endo has the capacity to form apatite and possess an apical sealing ability better than CRCS. Early calcium-hydroxide and C-S-H gel formation promote the nucleation of apatite deposits. The formation of apatite improve the apical sealing.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
