Aster yellows (AY) phytoplasmas have been reported for the first time in Serbia in carrots showing yellows symptoms (Duduk et al., Bulletin of Insectology 60, 341-342. 2007). To prevent further dangerous spreading of the disease, identification of the insect species possibly vectoring this phytoplasma and detection insect-carried phytoplasmas were carried out. During 2007 and 2008 leafhoppers were trapped from the beginning of April to the end of October, in two sites in the South Bačka region of Serbia, where phytoplasma associated diseases were reported although in low percentages (about 3%). Adult leafhoppers were sampled at two-week intervals, in carrot fields and weeds nearby, by using double-sided, yellow sticky traps (25x10) and by sweep netting in both sites. Leafhoppers were stored in 96% ethanol and identified before extraction of nucleic acids. Phytoplasma detection in identified species was carried out by nested PCR-RFLP assays on 16S ribosomal gene. The presence of four known vectors of AY phytoplasmas was recorded: Anaceratagallia laevis (Ribaut), Macrosteles laevis (Ribaut), M. quadripunctulatus (Kirschbaum) and M. sexnotatus (Fallen). Other six leafhopper species, which are not known, according to literature, as AY vectors i.e. Anaceratagallia ribauti (Ossiannilsson), A. venosa (Fourcroy), Empoasca spp., Scaphoideus titanus (Ball), Psammotettix alienus (Dahlbom), P. striatus (Linnaeus) were identified. The other five identified species, Eupteryx mellissae (Curtis), Ophiola decumana (Kontkanen), Psammotettix confinis (Dahlbom), P. notatus (Melichar) and Errastanus ocellaris (Fallén), are not known described as vectors. AY phytoplasmas were detected in the following vector and non-vector leafhopper species: A. laevis, A. ribauti, M. quadripunctulatus, M. sexnotatus, O. decumana and P. confinis. Transmission experiments with previously unreported vector species are in progress to provide evidence of their vector abilities.
Identifying potential phytoplasma vectors in infected carrot fields in Serbia / Drobnjaković T.; Perić P.; Marčić D.; Picciau L.; Alma A.; Mitrović J.; Duduk B.; Bertaccini A.. - STAMPA. - (2010), pp. 49-49. (Intervento presentato al convegno Current status and perspectives of phytoplasma disease research and management. tenutosi a Sitges, Spain nel February 1st and 2nd, 2010).
Identifying potential phytoplasma vectors in infected carrot fields in Serbia.
DUDUK, BOJAN;BERTACCINI, ASSUNTA
2010
Abstract
Aster yellows (AY) phytoplasmas have been reported for the first time in Serbia in carrots showing yellows symptoms (Duduk et al., Bulletin of Insectology 60, 341-342. 2007). To prevent further dangerous spreading of the disease, identification of the insect species possibly vectoring this phytoplasma and detection insect-carried phytoplasmas were carried out. During 2007 and 2008 leafhoppers were trapped from the beginning of April to the end of October, in two sites in the South Bačka region of Serbia, where phytoplasma associated diseases were reported although in low percentages (about 3%). Adult leafhoppers were sampled at two-week intervals, in carrot fields and weeds nearby, by using double-sided, yellow sticky traps (25x10) and by sweep netting in both sites. Leafhoppers were stored in 96% ethanol and identified before extraction of nucleic acids. Phytoplasma detection in identified species was carried out by nested PCR-RFLP assays on 16S ribosomal gene. The presence of four known vectors of AY phytoplasmas was recorded: Anaceratagallia laevis (Ribaut), Macrosteles laevis (Ribaut), M. quadripunctulatus (Kirschbaum) and M. sexnotatus (Fallen). Other six leafhopper species, which are not known, according to literature, as AY vectors i.e. Anaceratagallia ribauti (Ossiannilsson), A. venosa (Fourcroy), Empoasca spp., Scaphoideus titanus (Ball), Psammotettix alienus (Dahlbom), P. striatus (Linnaeus) were identified. The other five identified species, Eupteryx mellissae (Curtis), Ophiola decumana (Kontkanen), Psammotettix confinis (Dahlbom), P. notatus (Melichar) and Errastanus ocellaris (Fallén), are not known described as vectors. AY phytoplasmas were detected in the following vector and non-vector leafhopper species: A. laevis, A. ribauti, M. quadripunctulatus, M. sexnotatus, O. decumana and P. confinis. Transmission experiments with previously unreported vector species are in progress to provide evidence of their vector abilities.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
