Lipogenesis is a complex process mainly influenced by genes coding for enzymes involved in de novo fatty acids synthesis. Changes in mRNA abundance of these genes have been associated to obesity related traits but the genetic regulation of fat deposition is not completely known. The variability at DNA and mRNA level of FASN (Fatty Acid Sinthase), ACL (ATP Citrate Lyase) and ACACA (Acetyl CoA Carboxylase) genes, involved in key steps of fatty acids metabolism, was investigated in porcine skeletal muscle and fat tissues. We genotyped Italian Duroc (IDU) and Italian Large White (ILW) pigs both for the SNP T265C of FASN gene (Munoz et al 2003) and the SNP T2956C of ACL gene that we newly identified by in silico analisys and sequencing. We localized ACL gene on SSC12 (RH panel, LOD: 11.49 with SW943 marker) where FASN and ACACA genes map and where QTLs for fat related traits were detected. The mRNA level of the three genes was determined in skeletal muscle and fat tissues samples of ILW and IDU pigs. Differential expression between breeds (FASN P=0.057; ACL P=0.021; ACACA P=0.041) only in skeletal muscle tissue was observed and IDU pigs showed the higher level. Studies are in progress to investigate on relationship between DNA variation and gene expression. The transcription of FASN, ACL and ACACA genes was found to be regulated in both tissues and breeds suggesting that these genes can represent promising functional and positional candidates for fat deposition in meat and carcass.
Functional genomic approaches to study three porcine lipogenic genes / Comella M.; Zambonelli P; Braglia S.; Dall’Olio S.; Buttazzoni L.; Davoli R.. - ELETTRONICO. - (2009), pp. 29-29. (Intervento presentato al convegno Pig Genome III conference tenutosi a The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge nel 2nd – 4th November 2009).
Functional genomic approaches to study three porcine lipogenic genes
COMELLA, MARCO;ZAMBONELLI, PAOLO;BRAGLIA, SILVIA;DALL'OLIO, STEFANIA;DAVOLI, ROBERTA
2009
Abstract
Lipogenesis is a complex process mainly influenced by genes coding for enzymes involved in de novo fatty acids synthesis. Changes in mRNA abundance of these genes have been associated to obesity related traits but the genetic regulation of fat deposition is not completely known. The variability at DNA and mRNA level of FASN (Fatty Acid Sinthase), ACL (ATP Citrate Lyase) and ACACA (Acetyl CoA Carboxylase) genes, involved in key steps of fatty acids metabolism, was investigated in porcine skeletal muscle and fat tissues. We genotyped Italian Duroc (IDU) and Italian Large White (ILW) pigs both for the SNP T265C of FASN gene (Munoz et al 2003) and the SNP T2956C of ACL gene that we newly identified by in silico analisys and sequencing. We localized ACL gene on SSC12 (RH panel, LOD: 11.49 with SW943 marker) where FASN and ACACA genes map and where QTLs for fat related traits were detected. The mRNA level of the three genes was determined in skeletal muscle and fat tissues samples of ILW and IDU pigs. Differential expression between breeds (FASN P=0.057; ACL P=0.021; ACACA P=0.041) only in skeletal muscle tissue was observed and IDU pigs showed the higher level. Studies are in progress to investigate on relationship between DNA variation and gene expression. The transcription of FASN, ACL and ACACA genes was found to be regulated in both tissues and breeds suggesting that these genes can represent promising functional and positional candidates for fat deposition in meat and carcass.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
