The synthesis and physical-chemical characterization of the metal-ligand complex [Os(bpy)2(CO)(enIA)][OTf]2 (where enIA ) ethylenediamine iodoacetamide) with a sulfhydryl-specific functional group is described. The UV and visible absorption and luminescence emission, including lifetime and steady-state anisotropy, are reported for the free probe and the probe covalently linked to two test proteins. The spectroscopic properties of the probe are unaffected by chemical modification and subsequent covalent linkage to the proteins. The luminescence lifetime in aqueous buffer is approximately 200 ns and the limiting anisotropy is greater than 0.125, suggesting a potentially useful probe for biophysical investigations.
[Os(bpy)2(CO)(enIA)][OTf]2: A Novel Sulfhydryl-Specific Metal-Ligand Complex / Claudio Garino; Simona Ghiani; Roberto Gobetto; Carlo Nervi; Luca Salassa; Valentina Ancarani; P. Neyroz; Laurie Franklin; J. B. Alexander Ross; Eleanore Seibert|. - In: INORGANIC CHEMISTRY. - ISSN 0020-1669. - STAMPA. - 44:(2005), pp. 3875-3879.
[Os(bpy)2(CO)(enIA)][OTf]2: A Novel Sulfhydryl-Specific Metal-Ligand Complex
ANCARANI, VALENTINA;NEYROZ, PAOLO;
2005
Abstract
The synthesis and physical-chemical characterization of the metal-ligand complex [Os(bpy)2(CO)(enIA)][OTf]2 (where enIA ) ethylenediamine iodoacetamide) with a sulfhydryl-specific functional group is described. The UV and visible absorption and luminescence emission, including lifetime and steady-state anisotropy, are reported for the free probe and the probe covalently linked to two test proteins. The spectroscopic properties of the probe are unaffected by chemical modification and subsequent covalent linkage to the proteins. The luminescence lifetime in aqueous buffer is approximately 200 ns and the limiting anisotropy is greater than 0.125, suggesting a potentially useful probe for biophysical investigations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
