Capacitation is a process by which spermatozoa can acquire the possibility to fertilize an egg. There are many changes in the cell, regarding the plasmatic membrane, the protein's phosphorylation status, the activation of intracellular cascades and the preparation of the various structures of the cell to the acrosome reaction an subsequent fertilization. In sex sorting technique, spermatozoa undergo numerous changes, due to staining, separation and collection. The aim of this study was to verify if sex sorted semen undergoes changes in metabolism and functions that are similar to those observed during capacitation, focusing on protein-tyrosine phosphorylation, actin polymerization and Hsp70 rilocalization.
MODIFICAZIONI SIMIL-CAPACITATIVE NEGLI SPERMATOZOI SESSATI DI VERRO
BUCCI, DIEGO;SPINACI, MARCELLA;VALLORANI, CLAUDIA;TAMANINI, CARLO;GALEATI, GIOVANNA
2009
Abstract
Capacitation is a process by which spermatozoa can acquire the possibility to fertilize an egg. There are many changes in the cell, regarding the plasmatic membrane, the protein's phosphorylation status, the activation of intracellular cascades and the preparation of the various structures of the cell to the acrosome reaction an subsequent fertilization. In sex sorting technique, spermatozoa undergo numerous changes, due to staining, separation and collection. The aim of this study was to verify if sex sorted semen undergoes changes in metabolism and functions that are similar to those observed during capacitation, focusing on protein-tyrosine phosphorylation, actin polymerization and Hsp70 rilocalization.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
