Stemphylium vesicarium (Wallr.) E. Simmons is the causal agent of several plant diseases as well Brown Spot of Pear (BSP), which is one of the most economically important fungal diseases in European pear-production areas. Moreover, conidia widespread from plant material infected by the pathogen can trigger respiratory allergy. We were aimed to provide the first available genome sequence of S. vesicarium.The resource is presented accompanied by genome properties.Genomic DNA was extracted from the monoconidial strain 1731a13FI1M3 isolated from pear, then de novo sequenced by shotgun on Illumina Miseq platform v3. 1,127 contigs were assembled and a total assembly length of 38.66 Mb were obtained. The size was similar to those of Stemphylium lycopersici,the only other genome currently available for the Stemphilium genus. Gene prediction resulted in 12,309 putative genes.A primary functional annotation provided information about Orthologous Groups, Gene Ontology terms, KEGG pathways, and SMART/Pfam domains for each group. Furthermore, combined prediction of transmembrane topology and signal peptide were carried out, and genome sequence was also analyzed for the automatic genomic identification and analysis of biosynthetic gene clusters.Among predicted genes it was also possible to identify several orthologues required in other Ascomycota as related to: pathogenesis or full virulence on plants, signal transduction, cell respiration.Furthermore, the annotation was able to detect the orthologues of alt a 1 major and alt a 7 minor A. alternata allergens. The availability of this genome opens a new scenario in the investigation of S.vesicarium lifestyle and molecular plant/pathogen interaction, and could be fundamental to design more effective and sustainable fungicides management strategies to control BSP and other plant diseases. The genome sequence has been deposited in the GenBank under the accession number:QXCR00000000 (BioProject: PRJNA470620, BioSample: SAMN09098503).
Genome sequence of Stemphylium vesicarium, the causal agent of Brown Spot disease of Pear / Katia Gazzetti, Elena Lavinia Diaconu, Irene Maja Nanni, Alessandro Ciriani, Marina Collina. - ELETTRONICO. - (2020), pp. 1-1. (Intervento presentato al convegno 15th European Conference on Fungal Genetics tenutosi a Roma (Italia) nel 17-20 febbraio 2020).
Genome sequence of Stemphylium vesicarium, the causal agent of Brown Spot disease of Pear
Katia Gazzetti;Irene Maja Nanni;Alessandro Ciriani;Marina Collina
2020
Abstract
Stemphylium vesicarium (Wallr.) E. Simmons is the causal agent of several plant diseases as well Brown Spot of Pear (BSP), which is one of the most economically important fungal diseases in European pear-production areas. Moreover, conidia widespread from plant material infected by the pathogen can trigger respiratory allergy. We were aimed to provide the first available genome sequence of S. vesicarium.The resource is presented accompanied by genome properties.Genomic DNA was extracted from the monoconidial strain 1731a13FI1M3 isolated from pear, then de novo sequenced by shotgun on Illumina Miseq platform v3. 1,127 contigs were assembled and a total assembly length of 38.66 Mb were obtained. The size was similar to those of Stemphylium lycopersici,the only other genome currently available for the Stemphilium genus. Gene prediction resulted in 12,309 putative genes.A primary functional annotation provided information about Orthologous Groups, Gene Ontology terms, KEGG pathways, and SMART/Pfam domains for each group. Furthermore, combined prediction of transmembrane topology and signal peptide were carried out, and genome sequence was also analyzed for the automatic genomic identification and analysis of biosynthetic gene clusters.Among predicted genes it was also possible to identify several orthologues required in other Ascomycota as related to: pathogenesis or full virulence on plants, signal transduction, cell respiration.Furthermore, the annotation was able to detect the orthologues of alt a 1 major and alt a 7 minor A. alternata allergens. The availability of this genome opens a new scenario in the investigation of S.vesicarium lifestyle and molecular plant/pathogen interaction, and could be fundamental to design more effective and sustainable fungicides management strategies to control BSP and other plant diseases. The genome sequence has been deposited in the GenBank under the accession number:QXCR00000000 (BioProject: PRJNA470620, BioSample: SAMN09098503).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
