Abstract. Regulated promoters have a pivotal role in synthetic genetic program. To allow the rational design of this part we synthesized a library of operator sequences for LacI, TetR, cI and LexA repressor proteins. Single sequences were cloned as BioBricks (1) to allow their standard assembly with constitutive promoters. Thus, promoter transcriptional strength and repressor binding affinity can be independently fixed. A simple procedure was established to characterize the regulated promoter. These parts are expected to be a benefit in many Synthetic Biology applications.
Standard Synthetic Operator Sequences for the Rational Design of Regulated Promoters / F Ceroni; B Angelici; S Furini; G Mariotta; E Giordano; S Cavalcanti. - STAMPA. - (2009), pp. 117-118. (Intervento presentato al convegno IET Conference on Synthetic Biology, Systems Biology and Bionformatics University of Cambridge - UK tenutosi a West Road - University of Cambridge United Kingdom nel 23-25 March 2009).
Standard Synthetic Operator Sequences for the Rational Design of Regulated Promoters
CERONI, FRANCESCA;GIORDANO, EMANUELE DOMENICO;CAVALCANTI, SILVIO
2009
Abstract
Abstract. Regulated promoters have a pivotal role in synthetic genetic program. To allow the rational design of this part we synthesized a library of operator sequences for LacI, TetR, cI and LexA repressor proteins. Single sequences were cloned as BioBricks (1) to allow their standard assembly with constitutive promoters. Thus, promoter transcriptional strength and repressor binding affinity can be independently fixed. A simple procedure was established to characterize the regulated promoter. These parts are expected to be a benefit in many Synthetic Biology applications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
