Grape vine (Vitis vinifera L.) is one of the most cultivated plants in the world. Regular consumption of grape products is associated with a reduction in the onset of chronic-degenerative diseases. In the European Union most of the harvest is used by the wine industry, generating a large amount of grape processing by-products. Pomace, seeds and stalks are the main processing residues obtained during the winemaking process and represent matrices that could be exploited for sustainable recycling. Aim of this multidisciplinary research project is the extraction, identification and quantitation of bioactive compounds in grape processing by-products, the evaluation of the biological effects of bioactive-enriched extracts and the mapping of the identified bioactive substances by comparing the profiles of different plant parts (pomace, seeds and stalks) and grape cultivars (Albana and Sangiovese). In order to obtain bioactive-enriched samples exploiting eco-sustainable technologies, extraction procedures were developed by means of solid/liquid-, ultrasound- and microwave-assisted extraction (SLE, UAE, MAE). An original LC-MS/MS methodology has been set up, fully validated and applied to the extracts in order to obtain a detailed quali-quantitative profiling of several compound classes (flavonoids, phenolic acids, procyanidins, stilbenoids). Moreover, all the extracts were evaluated for their therapeutic potential on gastrointestinal diseases by exploiting ex-vivo animal tissues, and then correlated to their quali-quantitative composition by computational approaches based on molecular networks. The comparative characterisation of bioactive compounds present in grape by-products could be the basis for the valorisation and integrated exploitation of this cheap and easily available alternative source in the nutraceutical, cosmeceutical and pharmaceutical fields. This research was funded by “Fondazione Cassa di Risparmio di Imola” (Project code: 065.0318-2018.0050; Call year: 2017).
Characterisation of bioactive compounds in wine by-products: toward the valorisation of sustainable resources
Camilla Marasca;Irene Brusa;Chiara Cabrelle;Michele Protti;Matteo Micucci;Laura Mercolini;Marinella Roberti;Roberta Budriesi;Maurizio Recanatini
2019
Abstract
Grape vine (Vitis vinifera L.) is one of the most cultivated plants in the world. Regular consumption of grape products is associated with a reduction in the onset of chronic-degenerative diseases. In the European Union most of the harvest is used by the wine industry, generating a large amount of grape processing by-products. Pomace, seeds and stalks are the main processing residues obtained during the winemaking process and represent matrices that could be exploited for sustainable recycling. Aim of this multidisciplinary research project is the extraction, identification and quantitation of bioactive compounds in grape processing by-products, the evaluation of the biological effects of bioactive-enriched extracts and the mapping of the identified bioactive substances by comparing the profiles of different plant parts (pomace, seeds and stalks) and grape cultivars (Albana and Sangiovese). In order to obtain bioactive-enriched samples exploiting eco-sustainable technologies, extraction procedures were developed by means of solid/liquid-, ultrasound- and microwave-assisted extraction (SLE, UAE, MAE). An original LC-MS/MS methodology has been set up, fully validated and applied to the extracts in order to obtain a detailed quali-quantitative profiling of several compound classes (flavonoids, phenolic acids, procyanidins, stilbenoids). Moreover, all the extracts were evaluated for their therapeutic potential on gastrointestinal diseases by exploiting ex-vivo animal tissues, and then correlated to their quali-quantitative composition by computational approaches based on molecular networks. The comparative characterisation of bioactive compounds present in grape by-products could be the basis for the valorisation and integrated exploitation of this cheap and easily available alternative source in the nutraceutical, cosmeceutical and pharmaceutical fields. This research was funded by “Fondazione Cassa di Risparmio di Imola” (Project code: 065.0318-2018.0050; Call year: 2017).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
