The use of molecular markers in functional genes as tool to characterize the genetic diversity in livestock populations can provide new opportunities for the identification of putative alleles of economic value. The aim of this study was to investigate on the genetic diversity among eleven pig breeds by typing SNPs in 23 genes coding for protein. DNA samples of five Italian native pig breeds (Calabrese, Casertana, Cinta Senese, Mora Romagnola and Nero Siciliano) and six cosmopolite breeds (Italian Large White, Italian Landrace, Italian Duroc, Belgian Landrace, Hampshire and Piétrain) were used. A sample of Meishan breed was also included. Allele frequencies, heterozygosity, Fst, Fis, Fit, and Reynold’s genetic distances were calculated and Neighbour-Joining tree with bootstrap resampling was obtained. The average expected heterozygosity over all loci ranged from 0.187 in Mora Romagnola to 0.342 in Italian Large White. Mora Romagnola and Meishan breeds showed the lowest within-population diversity. The results obtained for SNPs were compared with those produced previously in Pig Biodiversity project that analysed DNA samples of the same breeds of the present study, except for Mora Romagnola, using microsatellites markers. The SNP marker-based regression of heterozygosity vs distance from each population to origin produced a similar trend to that obtained with microsatellites. This analysis indicates that the founder expected heterozygosity is 0.382 for SNPs and 0.773 for microsatellites.
SNPs and microsatellite markers for genetic diversity study in Italian pig breeds / Davoli R.; Zambonelli P.; San-Cristobal M.; Scotti E.; Fontanesi L.; Colombo M.; Dall’Olio S.; Braglia S.; Russo V.. - ELETTRONICO. - (2008), pp. 46-53. (Intervento presentato al convegno 6th International Symposium on the Mediterranean Pig tenutosi a Messina - Capo d’Orlando (ME), Italy nel October 11-13, 2007).
SNPs and microsatellite markers for genetic diversity study in Italian pig breeds
DAVOLI, ROBERTA;ZAMBONELLI, PAOLO;SCOTTI, EMILIO;FONTANESI, LUCA;COLOMBO, MICHELA;DALL'OLIO, STEFANIA;BRAGLIA, SILVIA;RUSSO, VINCENZO
2008
Abstract
The use of molecular markers in functional genes as tool to characterize the genetic diversity in livestock populations can provide new opportunities for the identification of putative alleles of economic value. The aim of this study was to investigate on the genetic diversity among eleven pig breeds by typing SNPs in 23 genes coding for protein. DNA samples of five Italian native pig breeds (Calabrese, Casertana, Cinta Senese, Mora Romagnola and Nero Siciliano) and six cosmopolite breeds (Italian Large White, Italian Landrace, Italian Duroc, Belgian Landrace, Hampshire and Piétrain) were used. A sample of Meishan breed was also included. Allele frequencies, heterozygosity, Fst, Fis, Fit, and Reynold’s genetic distances were calculated and Neighbour-Joining tree with bootstrap resampling was obtained. The average expected heterozygosity over all loci ranged from 0.187 in Mora Romagnola to 0.342 in Italian Large White. Mora Romagnola and Meishan breeds showed the lowest within-population diversity. The results obtained for SNPs were compared with those produced previously in Pig Biodiversity project that analysed DNA samples of the same breeds of the present study, except for Mora Romagnola, using microsatellites markers. The SNP marker-based regression of heterozygosity vs distance from each population to origin produced a similar trend to that obtained with microsatellites. This analysis indicates that the founder expected heterozygosity is 0.382 for SNPs and 0.773 for microsatellites.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
