This study was conducted to identify the incidence of a chickpea disease consisting in phyllody, virescence and proliferation, its association with phytoplasmas and its insect vector(s). The presence of phytoplasmas in leafhopper species and chickpea plants was verified by nested PCR amplification of 16S rDNA. Orosius albicinctus, Circulifer haematoceps and Neoaliturus fenestratus resulted phytoplasma positive. The sequence of the detected phytoplasma strain exhibited the maximum nucleotide identity (99.9-100%) with peanut witches’ broom phytoplasmas, 16SrII-A subgroup.
Molecular identification and characterization of phytoplasmas in insect vectors of chickpea phyllody disease in Punjab, Pakistan / Ahmad J.N., M.Z. Sharif, S.J.N. Ahmad, M. Tahir, A. Bertaccini. - In: PHYTOPATHOGENIC MOLLICUTES. - ISSN 2249-4669. - STAMPA. - 9:1(2019), pp. 105-106. [10.5958/2249-4677.2019.00053.7]
Molecular identification and characterization of phytoplasmas in insect vectors of chickpea phyllody disease in Punjab, Pakistan
A. Bertaccini
2019
Abstract
This study was conducted to identify the incidence of a chickpea disease consisting in phyllody, virescence and proliferation, its association with phytoplasmas and its insect vector(s). The presence of phytoplasmas in leafhopper species and chickpea plants was verified by nested PCR amplification of 16S rDNA. Orosius albicinctus, Circulifer haematoceps and Neoaliturus fenestratus resulted phytoplasma positive. The sequence of the detected phytoplasma strain exhibited the maximum nucleotide identity (99.9-100%) with peanut witches’ broom phytoplasmas, 16SrII-A subgroup.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
