The development of methods for culturing phytoplasmas in artificial media suggested the evaluation of strategies to produce antibodies against a phytoplasma target. To this purpose, an aster yellows phytoplasma isolate obtained from field collected infected grapevine was used for the production of a polyclonal antiserum. It was then tested by ELISA and IFAS methods in order to check and evaluate its suitability for phytoplasma detection in plant samples.
Preliminary evaluation of the use of an antiserum obtained from phytoplasma culture / Contaldo N., I. Freo, C. Lucchese, L. Formica, A. Bertaccini. - In: PHYTOPATHOGENIC MOLLICUTES. - ISSN 2249-4669. - STAMPA. - 9:1(2019), pp. 201-202. [10.5958/2249-4677.2019.00101.4]
Preliminary evaluation of the use of an antiserum obtained from phytoplasma culture
Contaldo N.
;C. Lucchese;A. Bertaccini
2019
Abstract
The development of methods for culturing phytoplasmas in artificial media suggested the evaluation of strategies to produce antibodies against a phytoplasma target. To this purpose, an aster yellows phytoplasma isolate obtained from field collected infected grapevine was used for the production of a polyclonal antiserum. It was then tested by ELISA and IFAS methods in order to check and evaluate its suitability for phytoplasma detection in plant samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
