The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.
Fluorescence lifetime imaging of intracellular magnesium content in live cells / Sargenti A.; Candeo A.; Farruggia G.; D'Andrea C.; Cappadone C.; Malucelli E.; Valentini G.; Taroni P.; Iotti S.. - In: ANALYST. - ISSN 0003-2654. - STAMPA. - 144:6(2019), pp. 1876-1880. [10.1039/c8an02379d]
Fluorescence lifetime imaging of intracellular magnesium content in live cells
Sargenti A.Writing – Original Draft Preparation
;Farruggia G.Investigation
;Cappadone C.Investigation
;Malucelli E.Data Curation
;Iotti S.
Conceptualization
2019
Abstract
The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
