The morphological arrangement of scaffold is fundamental to drive cell growth and regeneration of collagen extracellular matrix. Electrospinning is a very promising technique to produce nanofibrous scaffolds able to guide cells in the regeneration of injured tissues. We developed a resorbable electrospun scaffold mimicking the hierarchical structure of tendons and ligaments. The aim of the present study was to investigate if hierarchically structured poly(L-lactic acid) (PLLA) and collagen (Coll) electrospun scaffolds can induce morphological modifications in human fibroblasts, while cultured in static and dynamic conditions. Different imaging techniques such as high-resolution x-ray computed tomography (XCT), scanning electron microscopy (SEM), fluorescence microscopy and histology were combined to assess the potential of such scaffolds.
Hierarchical electrospun tendon/ligament-inspired scaffolds can modify fibroblasts morphology in static and dynamic cultures / A. Sensini, L. Cristofolini, A. Zucchelli, M. L. Focarete, C. Gualandi, M. Fichera, A. De Mori, A. P. Kao, M. Roldo, G. Blunn, G. Tozzi. - ELETTRONICO. - (2019). (Intervento presentato al convegno ESB-ITA 2019 tenutosi a Bologna nel 30/09-1/10/2019).
Hierarchical electrospun tendon/ligament-inspired scaffolds can modify fibroblasts morphology in static and dynamic cultures
A. Sensini;L. Cristofolini;A. Zucchelli;M. L. Focarete;C. Gualandi;
2019
Abstract
The morphological arrangement of scaffold is fundamental to drive cell growth and regeneration of collagen extracellular matrix. Electrospinning is a very promising technique to produce nanofibrous scaffolds able to guide cells in the regeneration of injured tissues. We developed a resorbable electrospun scaffold mimicking the hierarchical structure of tendons and ligaments. The aim of the present study was to investigate if hierarchically structured poly(L-lactic acid) (PLLA) and collagen (Coll) electrospun scaffolds can induce morphological modifications in human fibroblasts, while cultured in static and dynamic conditions. Different imaging techniques such as high-resolution x-ray computed tomography (XCT), scanning electron microscopy (SEM), fluorescence microscopy and histology were combined to assess the potential of such scaffolds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
