An HPLC method for the determination of the main organic acids and sugars in friuts juices is proposed. Nine acids (includind oxalic, citric, malic, quinic, galacturonic, ascorbic, succinic and fumaric acid) and three sugars (sucrose, glucose and fructose) were separeted by ion-exclusion chromatography using a resin-based Aminex HPX 87H column after a sample clean-up with Sax cartridges. In spite of the suitable chromatographyc conditions, quantification of ascorbic and dehydroascorbic acid was affected by on-column degradation of the former into the latter. For all the other analytes the mothod showed a good precision and linearity and, as an application, eigtheen commercial juices from 4 different friut were tested.
Optimization of the determination of organic acid and sugars in fruit juices by ion-exclusion liquid chromatography
CHINNICI, FABIO;SPINABELLI, UMBERTO;RIPONI, CLAUDIO;AMATI, AURELIANO
2005
Abstract
An HPLC method for the determination of the main organic acids and sugars in friuts juices is proposed. Nine acids (includind oxalic, citric, malic, quinic, galacturonic, ascorbic, succinic and fumaric acid) and three sugars (sucrose, glucose and fructose) were separeted by ion-exclusion chromatography using a resin-based Aminex HPX 87H column after a sample clean-up with Sax cartridges. In spite of the suitable chromatographyc conditions, quantification of ascorbic and dehydroascorbic acid was affected by on-column degradation of the former into the latter. For all the other analytes the mothod showed a good precision and linearity and, as an application, eigtheen commercial juices from 4 different friut were tested.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
