Tetraploid wheat species, with his two wild and cultivated subspecies T. turgidum ssp. dicoccoides and ssp. durum, is a cornerstone of the wheat polyploidy complex. Durum wheat is one of the most important cereals for Mediterranean and other countries with semi-arid environments. Additionally, tetraploid wheat genetic resources are valuable for the genetic improvement of common wheat. Until now, durum wheat molecular genetics and genomics have largely relied on common wheat molecular tools. In 2016, following the assembly of the T. dicoccoides genome, an international consortium has generated a high-quality reference sequence of the modern durum wheat cultivar Svevo based on the NR-Gene DeNovoMAGICTM assembly pipeline. Both genomes are anchored to a common high-density genetic map based on Svevo × Zavitan RIL population and merged into super-scaffolds using Hi-C data. The Svevo assembly scaffolds span 10.5 Gb, with 95% of the scaffolds anchored and 90% oriented. Additionally 15 high-density linkage maps based on the 90K SNP array are used to further anchoring and to explore the QTLome of tetraploid wheat through GWAS and meta-QTL analysis. Here, we present preliminary results on the use of the two tetraploid wheat genome assemblies to explore the gene content of QTL regions relevant for root system architecture, for a grain yield QTL located on chromosome 3B (QYld.idw-3B) and for a major locus for resistance to Soil-borne mosaic virus (Sbm2). The access to the two tetraploid genome sequences provides unprecedented opportunities to accellerate the positional cloning of these as well as other QTLs.
P0845: Leveraging the Assembly of the Durum Wheat Genome cv. Svevo
M. Maccaferri;
2017
Abstract
Tetraploid wheat species, with his two wild and cultivated subspecies T. turgidum ssp. dicoccoides and ssp. durum, is a cornerstone of the wheat polyploidy complex. Durum wheat is one of the most important cereals for Mediterranean and other countries with semi-arid environments. Additionally, tetraploid wheat genetic resources are valuable for the genetic improvement of common wheat. Until now, durum wheat molecular genetics and genomics have largely relied on common wheat molecular tools. In 2016, following the assembly of the T. dicoccoides genome, an international consortium has generated a high-quality reference sequence of the modern durum wheat cultivar Svevo based on the NR-Gene DeNovoMAGICTM assembly pipeline. Both genomes are anchored to a common high-density genetic map based on Svevo × Zavitan RIL population and merged into super-scaffolds using Hi-C data. The Svevo assembly scaffolds span 10.5 Gb, with 95% of the scaffolds anchored and 90% oriented. Additionally 15 high-density linkage maps based on the 90K SNP array are used to further anchoring and to explore the QTLome of tetraploid wheat through GWAS and meta-QTL analysis. Here, we present preliminary results on the use of the two tetraploid wheat genome assemblies to explore the gene content of QTL regions relevant for root system architecture, for a grain yield QTL located on chromosome 3B (QYld.idw-3B) and for a major locus for resistance to Soil-borne mosaic virus (Sbm2). The access to the two tetraploid genome sequences provides unprecedented opportunities to accellerate the positional cloning of these as well as other QTLs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
