A invertebrate DNA microarray targeting ten species common in the Mediterranean was developed within the framework of the FP6 STREP FISH& CHIPS. Based on sequence variation of the 16S rDNA gene, we designed species-specific probes that were used to construct a DNA chip prototype for the identification of invertebrate (i.e. molluscans and crustaceans) preys of demersal fishes in the Adriatic Sea. The constructed invertebrate DNA chip represents a technological advance towards the wide use of rapid and high-resolution molecular tools to assess structure and changes of marine ecosystems such as the monitoring of invasive species and of re-emerging pathogens in ballast waters.
DEVELOPMENT OF HIGH-RESOLUTION MOLECULAR TECHNOLOGIES TO INVESTIGATE ECOSYSTEM CHANGES IN THE SOUTHERN EUROPEAN SEAS: THE PROTOTYPING OF DNA CHIPS TO IDENTIFY INVERTEBRATE PREY SPECIES IN MARINE FOOD WEBS OF ADRIATIC SEA / A. Cariani; M. Landi; F. Bertasi; M. Colangelo; V.U. Ceccherelli; F. Tinti; S. Chitipothu; D. Blohm. - In: RAPPORTS ET PROCÈS VERBAUX DES RÉUNIONS - COMMISSION INTERNATIONALE POUR L'EXPLORATION SCIENTIFIQUE DE LA MER MÉDITERRANÉE. - ISSN 0373-434X. - ELETTRONICO. - 38:(2007), pp. 444-444. (Intervento presentato al convegno 38th CIESM Congress tenutosi a Istanbul (Turkey) nel 9-13 April 2007).
DEVELOPMENT OF HIGH-RESOLUTION MOLECULAR TECHNOLOGIES TO INVESTIGATE ECOSYSTEM CHANGES IN THE SOUTHERN EUROPEAN SEAS: THE PROTOTYPING OF DNA CHIPS TO IDENTIFY INVERTEBRATE PREY SPECIES IN MARINE FOOD WEBS OF ADRIATIC SEA
CARIANI, ALESSIA;LANDI, MONICA;COLANGELO, MARINA ANTONIA;TINTI, FAUSTO;
2007
Abstract
A invertebrate DNA microarray targeting ten species common in the Mediterranean was developed within the framework of the FP6 STREP FISH& CHIPS. Based on sequence variation of the 16S rDNA gene, we designed species-specific probes that were used to construct a DNA chip prototype for the identification of invertebrate (i.e. molluscans and crustaceans) preys of demersal fishes in the Adriatic Sea. The constructed invertebrate DNA chip represents a technological advance towards the wide use of rapid and high-resolution molecular tools to assess structure and changes of marine ecosystems such as the monitoring of invasive species and of re-emerging pathogens in ballast waters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
