Background: Polypodium leucotomos (PL) exerts potent antioxidant, photo-protective, and immune-modulatory activities. A reconstructed human epidermis (RHE) (Episkin) is a suitable model for the evaluation of acute UV-induced cell damage. No data regarding the photo-protective action of PL in this model are available. Purpose: We evaluated the effects of PL on the prevention of UVB-induced cell damage assessing sunburn cells, CPD formation, p53, Ki-67, p21 expression, and epidermal growth factor (EGF) production. Materials & Methods: RHE was incubated in standard conditions. PL was topically applied at the concentration of 2 mg/cm2, immediately before UVB exposition. UVB exposition (300 mJ/cm2) was performed using a dedicated UVB lamp. Irradiated samples without PL and non-irradiated samples were used as positive and negative controls. Expression of p53, p21, and Ki-67 was evaluated with immune-histochemical methods. CPD were measured using a monoclonal antibody. Results: PL significantly reduced sunburned cells (-80%) in comparison with positive control. PL significantly prevented the increase in EGF production at tested times. PL significantly reduced the p53 (-80%), p21 (-84%), and Ki-67 (-48%) positive cells. Finally, PL prevented the formation of CPD (0% vs. 20% positive cells). Conclusion: In this model, PL has shown to prevent UVB cell damage, the upregulation of proliferating proteins, and fully blocking the formation of CPD.
Protective effects of Polypodium leucotomos extract against UVB-induced damage in a model of reconstructed human epidermis / Torricelli, Paola; Fini, Milena; Fanti, Pier Alessandro; Dika, Emi; Milani, Massimo. - In: PHOTODERMATOLOGY, PHOTOIMMUNOLOGY & PHOTOMEDICINE. - ISSN 0905-4383. - STAMPA. - 33:3(2017), pp. 156-163. [10.1111/phpp.12297]
Protective effects of Polypodium leucotomos extract against UVB-induced damage in a model of reconstructed human epidermis
FINI, MILENA;FANTI, PIER ALESSANDRO;DIKA, EMI;
2017
Abstract
Background: Polypodium leucotomos (PL) exerts potent antioxidant, photo-protective, and immune-modulatory activities. A reconstructed human epidermis (RHE) (Episkin) is a suitable model for the evaluation of acute UV-induced cell damage. No data regarding the photo-protective action of PL in this model are available. Purpose: We evaluated the effects of PL on the prevention of UVB-induced cell damage assessing sunburn cells, CPD formation, p53, Ki-67, p21 expression, and epidermal growth factor (EGF) production. Materials & Methods: RHE was incubated in standard conditions. PL was topically applied at the concentration of 2 mg/cm2, immediately before UVB exposition. UVB exposition (300 mJ/cm2) was performed using a dedicated UVB lamp. Irradiated samples without PL and non-irradiated samples were used as positive and negative controls. Expression of p53, p21, and Ki-67 was evaluated with immune-histochemical methods. CPD were measured using a monoclonal antibody. Results: PL significantly reduced sunburned cells (-80%) in comparison with positive control. PL significantly prevented the increase in EGF production at tested times. PL significantly reduced the p53 (-80%), p21 (-84%), and Ki-67 (-48%) positive cells. Finally, PL prevented the formation of CPD (0% vs. 20% positive cells). Conclusion: In this model, PL has shown to prevent UVB cell damage, the upregulation of proliferating proteins, and fully blocking the formation of CPD.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
