Ligand-gated ion channels electrical behaviour and, in particular, their conductance are influenced by the binding between receptive sites on channel surface and specific molecules. These channels, inserted in biomimetic membranes and in presence of a proper electronic system for acquiring and elaborating the electrical signal, could give us the possibility of detecting and quantifying concentrations of specific molecules in complex mixtures from ionic currents across the membrane. In this paper we propose an algorithm for extracting the information of target (gating) molecule concentration from an estimation of single-channel open-state probability (p) that, in turn, requires an estimation of the number of active channels.
Quantitative Detection by Single Ion Channel Event Statistics / F. Lodesani; M. Bennati; C. Fiegna; M. Tartagni. - STAMPA. - (2007), pp. 563-566. (Intervento presentato al convegno NANOTECH 2007 tenutosi a Santa Clara (US) nel 2007).
Quantitative Detection by Single Ion Channel Event Statistics
LODESANI, FRANCESCO;BENNATI, MARCO;FIEGNA, CLAUDIO;TARTAGNI, MARCO
2007
Abstract
Ligand-gated ion channels electrical behaviour and, in particular, their conductance are influenced by the binding between receptive sites on channel surface and specific molecules. These channels, inserted in biomimetic membranes and in presence of a proper electronic system for acquiring and elaborating the electrical signal, could give us the possibility of detecting and quantifying concentrations of specific molecules in complex mixtures from ionic currents across the membrane. In this paper we propose an algorithm for extracting the information of target (gating) molecule concentration from an estimation of single-channel open-state probability (p) that, in turn, requires an estimation of the number of active channels.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.