Grapevine pinot gris virus (GPGV), a new trichovirus , was recently suggested to be associated with symptoms of leaf chlorotic mottling and deformations observed on grapevine plants of cv. Pinot gris in Trentino. GPGV has a genomic organization identical to that of grapevine inner berry necrosis virus (GINV), which is known to be transmitted by the erineum or leaf blister grape mite, Columeris vitis.This mite species is quite common in infected vineyards so that 2 experimental trails were carried out in order to investigate its possible involvment in GPGV transmission. C. vitis was transferred from galls of virus-free leaves to GPGV-infected grapevine leaves for the acquisition-access feeding. After various periods, some mites were analyzed by RT-PCR for the presence of GPGV and some were moved to virus-free grapevine seedlings for virus transmission. The second transmission experiment consisted in collecting C. vitis directly from galls of GPGV-infected leaves, then proceeding, as described above, with RT-PCR analysis and placing the mites on virus-free seedlings. Results demonstrated the presence of the virus in 2 pools of eriophyids analyzed, whereas no amplification was obtained from seedlings used for transmission trials. Parallel experiments were also conducted on pools or single individuals of C. vitis to characterize their ITS1 region, amplified using the specific primer pair 18S and 5.8S rev.
Transmission trials of grapevine pinot gris virus by the eriophyoid mite Columeris vitis
BEBER, ROBERTA;POGGI POLLINI, CARLO;RATTI, CLAUDIO;TERLIZZI, FEDERICA
2013
Abstract
Grapevine pinot gris virus (GPGV), a new trichovirus , was recently suggested to be associated with symptoms of leaf chlorotic mottling and deformations observed on grapevine plants of cv. Pinot gris in Trentino. GPGV has a genomic organization identical to that of grapevine inner berry necrosis virus (GINV), which is known to be transmitted by the erineum or leaf blister grape mite, Columeris vitis.This mite species is quite common in infected vineyards so that 2 experimental trails were carried out in order to investigate its possible involvment in GPGV transmission. C. vitis was transferred from galls of virus-free leaves to GPGV-infected grapevine leaves for the acquisition-access feeding. After various periods, some mites were analyzed by RT-PCR for the presence of GPGV and some were moved to virus-free grapevine seedlings for virus transmission. The second transmission experiment consisted in collecting C. vitis directly from galls of GPGV-infected leaves, then proceeding, as described above, with RT-PCR analysis and placing the mites on virus-free seedlings. Results demonstrated the presence of the virus in 2 pools of eriophyids analyzed, whereas no amplification was obtained from seedlings used for transmission trials. Parallel experiments were also conducted on pools or single individuals of C. vitis to characterize their ITS1 region, amplified using the specific primer pair 18S and 5.8S rev.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
