Extensive metabolic profiles of body fluids, obtained by nuclear magnetic resonance (NMR) spectroscopic or mass spectroscopic data, can provide information to deduce the effects of many different factors on biological processes. In particular, genetic factors can determine differences in physiological processes that are captured by metabolomics or metabonomics analyses. In this study we compared metabonomic profiles obtained from serum of two pig breeds (Italian Large White and Italian Duroc) with the aim to disentangle breed specific biological aspects. Twelve castrated males (6 Italian Large White and 6 Italian Duroc) were raised at the test station of the National Pig Breeders Association (ANAS) in the same conditions till they reached a live weight of about 160 kg. After a period of starvation, early in the morning pigs were transported all together at a commercial slaughterhouse, electrically stunned and jugulated within 10 minutes. Collected blood sample were processed after about 2 hours and serum was stored at -80°C till use. High Resolution Magic Angle Spinning-NMR serum spectra were recorded with a Bruker AVANCE spectrometer. Each sample was analysed in triplicate. Preliminary Principal Component Analysis of the obtained NMR spectra indicated that metabonomic analysis could be able to distinguish breed specific metabolic profiles. These results might represent useful information to disclose genetically determined regulatory pathways contributing to understand breed differences in biological processes indirectly linked with production traits.
A comparative serum metabonomic analysis between Italian Duroc and Italian Large White pigs
FONTANESI, LUCA;SCOTTI, EMILIO;DALL'OLIO, STEFANIA;
2013
Abstract
Extensive metabolic profiles of body fluids, obtained by nuclear magnetic resonance (NMR) spectroscopic or mass spectroscopic data, can provide information to deduce the effects of many different factors on biological processes. In particular, genetic factors can determine differences in physiological processes that are captured by metabolomics or metabonomics analyses. In this study we compared metabonomic profiles obtained from serum of two pig breeds (Italian Large White and Italian Duroc) with the aim to disentangle breed specific biological aspects. Twelve castrated males (6 Italian Large White and 6 Italian Duroc) were raised at the test station of the National Pig Breeders Association (ANAS) in the same conditions till they reached a live weight of about 160 kg. After a period of starvation, early in the morning pigs were transported all together at a commercial slaughterhouse, electrically stunned and jugulated within 10 minutes. Collected blood sample were processed after about 2 hours and serum was stored at -80°C till use. High Resolution Magic Angle Spinning-NMR serum spectra were recorded with a Bruker AVANCE spectrometer. Each sample was analysed in triplicate. Preliminary Principal Component Analysis of the obtained NMR spectra indicated that metabonomic analysis could be able to distinguish breed specific metabolic profiles. These results might represent useful information to disclose genetically determined regulatory pathways contributing to understand breed differences in biological processes indirectly linked with production traits.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
