The development of high throughput genomics (next generation sequencing and high throughput genotyping) and metabolomics platforms has opened new perspectives for the identification of the genetics factors affecting traits of biological relevance in all species, including production traits in farm animals. In pigs, benefits derived from the recent sequencing of the pig genome can be fully exploited by combining advanced genomics and metabolomics approaches. In this work we integrated several resources, experiments and data with the final aim to identify markers (DNA polymorphisms and metabolites) associated with production traits in Italian Large White pigs. High throughput genotyping was carried out using the Illumina Porcine60SNP BeadChip array and customized Golden Gate system on extreme and divergent pigs for back fat thickness (BFT) estimated breeding values (EBVs) (300-560 animals) and average daily gain (ADG) EBVs (360 pigs), chosen among a population of about 12,000 performance tested pigs. Next generation sequencing was carried out using the Ion Torrent PGM machine to identify single nucleotide polymorphisms (SNPs) from two reduced representation libraries developed from pooled genomic DNA constructed from 50 pigs with most positive and 50 pigs with most negative BFT EBVs, respectively. A total of 7,510,918 reads were produced and 447,031 SNPs were called, using stringent criteria. Genome wide association studies made it possible to identify a quite large number of significant SNPs affecting BFT, ADG and correlated traits. In addition, several genome regions containing significant SNPs for BFT were enriched of SNPs identified from the Next Generation Sequencing experiment. Metabolomics information was obtained from 800 performance tested pigs using a mass spectrometry (MS/MS) analytical pipeline to measure 180 blood plasma metabolites. Estimated heritability and correlation among all these parameters and production traits indicated that a few metabolites could be useful predictors of EBVs for production traits. All these data will be used to develop a first systems biology platform to understand the fine biological mechanisms affecting production traits in pigs.

Combined genomics and metabolomics approaches to identify markers associated with production traits in pigs.

FONTANESI, LUCA;DALL'OLIO, STEFANIA;FANELLI, FLAMINIA;SCOTTI, EMILIO;SCHIAVO, GIUSEPPINA;BERTOLINI, FRANCESCA;TASSONE, FRANCESCO;SAMORE', ANTONIA BIANCA;BOVO, SAMUELE;MAZZONI, GIANLUCA;GALIMBERTI, GIULIANO;CALO', DANIELA GIOVANNA;MARTELLI, PIER LUIGI;CASADIO, RITA;PAGOTTO, UBERTO;RUSSO, VINCENZO
2013

Abstract

The development of high throughput genomics (next generation sequencing and high throughput genotyping) and metabolomics platforms has opened new perspectives for the identification of the genetics factors affecting traits of biological relevance in all species, including production traits in farm animals. In pigs, benefits derived from the recent sequencing of the pig genome can be fully exploited by combining advanced genomics and metabolomics approaches. In this work we integrated several resources, experiments and data with the final aim to identify markers (DNA polymorphisms and metabolites) associated with production traits in Italian Large White pigs. High throughput genotyping was carried out using the Illumina Porcine60SNP BeadChip array and customized Golden Gate system on extreme and divergent pigs for back fat thickness (BFT) estimated breeding values (EBVs) (300-560 animals) and average daily gain (ADG) EBVs (360 pigs), chosen among a population of about 12,000 performance tested pigs. Next generation sequencing was carried out using the Ion Torrent PGM machine to identify single nucleotide polymorphisms (SNPs) from two reduced representation libraries developed from pooled genomic DNA constructed from 50 pigs with most positive and 50 pigs with most negative BFT EBVs, respectively. A total of 7,510,918 reads were produced and 447,031 SNPs were called, using stringent criteria. Genome wide association studies made it possible to identify a quite large number of significant SNPs affecting BFT, ADG and correlated traits. In addition, several genome regions containing significant SNPs for BFT were enriched of SNPs identified from the Next Generation Sequencing experiment. Metabolomics information was obtained from 800 performance tested pigs using a mass spectrometry (MS/MS) analytical pipeline to measure 180 blood plasma metabolites. Estimated heritability and correlation among all these parameters and production traits indicated that a few metabolites could be useful predictors of EBVs for production traits. All these data will be used to develop a first systems biology platform to understand the fine biological mechanisms affecting production traits in pigs.
ITALIAN JOURNAL OF ANIMAL SCIENCE
Fontanesi L.; Dall’Olio S.; Fanelli F.; Scotti E.; Schiavo G.; Bertolini F.; Tassone F.; Samoré A.B.; Bovo S; Mazzoni G.; Gallo M.; Buttazzoni L.; Galimberti G.; Calò D.G.; Martelli P.L.; Casadio R.; Pagotto U.; Russo V.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/395008
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