Determination of protein function is a key step for annotating new genomes and proteomes generated at ever increasing rate with the modern sequencing techniques. Gene and protein functions are usually described with the Gene Ontology (GO) that provides a controlled vocabulary of terms for defining Biological Processes (BP), Molecular Functions (MF) and Cellular Components (CC) (1). Owing to the experimental difficulties, computational tools are needed for extracting functional information from curated databases of protein sequences and structures and for generalizing it to new sequences (2). In order to predict the function of the 100,816 targets of the CAFA 2014 experiment, we implemented a pipeline that exploits different methods developed at the Bologna Biocomputing Group, including BAR+, BaCelLo, MemLoci, ENSEMBLE, BetAware, TPpred, SPEP, and GPIpred.
A pipeline for predicting the function of the AFP/CAFA 2014 targets at the Bologna Biocomputing Group
PROFITI, GIUSEPPE;MARTELLI, PIER LUIGI;CASADIO, RITA
2014
Abstract
Determination of protein function is a key step for annotating new genomes and proteomes generated at ever increasing rate with the modern sequencing techniques. Gene and protein functions are usually described with the Gene Ontology (GO) that provides a controlled vocabulary of terms for defining Biological Processes (BP), Molecular Functions (MF) and Cellular Components (CC) (1). Owing to the experimental difficulties, computational tools are needed for extracting functional information from curated databases of protein sequences and structures and for generalizing it to new sequences (2). In order to predict the function of the 100,816 targets of the CAFA 2014 experiment, we implemented a pipeline that exploits different methods developed at the Bologna Biocomputing Group, including BAR+, BaCelLo, MemLoci, ENSEMBLE, BetAware, TPpred, SPEP, and GPIpred.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
