Peptide dendrimers are a class of molecules that exhibit a large array of biological effects including antiviral activity. In this report, we analyzed the antiviral activity of the peptide-derivatized SB105-A10 dendrimer, which is a tetra-branched dendrimer synthetized on a lysine core, in activated peripheral blood mononuclear cells (PBMCs) that were challenged with reference and wild-type human immunodeficiency virus type 1 (HIV-1) strains. SB105-A10 inhibited infections by HIV-1 X4 and R5 strains, interfering with the early phases of the viral replication cycle. SB105-A10 targets heparan sulfate proteoglycans (HSPGs) and, importantly, the surface plasmon resonance (SPR) assay revealed that SB105-A10 strongly binds gp41 and gp120, most likely preventing HIV-1 attachment/entry through multiple mechanisms. Interestingly, the antiviral activity of SB105-A10 was also detectable in an organ-like structure of human cervicovaginal tissue, in which SB105-A10 inhibited the HIV-1ada R5 strain infection without altering the tissue viability. These results demonstrated the strong antiviral activity of SB105-A10 and suggest a potential microbicide use of this dendrimer to prevent the heterosexual transmission of HIV-1.

Peptide-derivatized SB105-A10 dendrimer inhibits the infectivity of R5 and X4 HIV-1 strains in primary PBMCs and cervicovaginal histocultures / Bon I; Lembo D; Rusnati M; Clò A; Morini S; Miserocchi A; Bugatti A; Grigolon S; Musumeci G; Landolfo S; Re MC; Gibellini D.. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 8:10(2013), pp. e76482.1-e76482.13. [10.1371/journal.pone.0076482]

Peptide-derivatized SB105-A10 dendrimer inhibits the infectivity of R5 and X4 HIV-1 strains in primary PBMCs and cervicovaginal histocultures.

BON, ISABELLA;Clò A;MORINI, SILVIA;MISEROCCHI, ANNA;MUSUMECI, GIUSEPPINA;RE, MARIA CARLA;GIBELLINI, DAVIDE
2013

Abstract

Peptide dendrimers are a class of molecules that exhibit a large array of biological effects including antiviral activity. In this report, we analyzed the antiviral activity of the peptide-derivatized SB105-A10 dendrimer, which is a tetra-branched dendrimer synthetized on a lysine core, in activated peripheral blood mononuclear cells (PBMCs) that were challenged with reference and wild-type human immunodeficiency virus type 1 (HIV-1) strains. SB105-A10 inhibited infections by HIV-1 X4 and R5 strains, interfering with the early phases of the viral replication cycle. SB105-A10 targets heparan sulfate proteoglycans (HSPGs) and, importantly, the surface plasmon resonance (SPR) assay revealed that SB105-A10 strongly binds gp41 and gp120, most likely preventing HIV-1 attachment/entry through multiple mechanisms. Interestingly, the antiviral activity of SB105-A10 was also detectable in an organ-like structure of human cervicovaginal tissue, in which SB105-A10 inhibited the HIV-1ada R5 strain infection without altering the tissue viability. These results demonstrated the strong antiviral activity of SB105-A10 and suggest a potential microbicide use of this dendrimer to prevent the heterosexual transmission of HIV-1.
2013
Peptide-derivatized SB105-A10 dendrimer inhibits the infectivity of R5 and X4 HIV-1 strains in primary PBMCs and cervicovaginal histocultures / Bon I; Lembo D; Rusnati M; Clò A; Morini S; Miserocchi A; Bugatti A; Grigolon S; Musumeci G; Landolfo S; Re MC; Gibellini D.. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 8:10(2013), pp. e76482.1-e76482.13. [10.1371/journal.pone.0076482]
Bon I; Lembo D; Rusnati M; Clò A; Morini S; Miserocchi A; Bugatti A; Grigolon S; Musumeci G; Landolfo S; Re MC; Gibellini D.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/391800
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