Glucose Biosensor Mediated by 1,2 Diferrocenylethane in a Sono-gel Composite Electrode

An amperometric glucose biosensor was constructed based on a renewable carbon composite sono-gel matrix incorporating 1,2-diferrocenylethane as electron transfer mediator between the electrode and the active site of glucose oxidase. The enzyme was immobilized on the electrode surface by cross-linking with glutaraldehyde and bovine serum albumin. The process parameters for the fabrication of the biosensor and the influence of various experimental conditions (i.e., pH, temperature, operating potential) were investigated. Cyclic voltammetry and amperometric measurements were used to study the response of the glucose sensor, which displayed fast response time and good reproducibility. The analytical performances and the apparent Michaelis-Menten constant of the biosensor were evaluated.