Thirty-one canine cutaneous nodules, diagnosed as mast cell tumors by histopathologic analysis on hematoxylin and eosin (HE) and toluidine blue stained sections, were used in this study to evaluate the immunohistochemical pattern of expression of KIT protein (CD117), a type III tyrosine kinase protein that is the receptor for stem cell factor (SCF), a cytokine that stimulates mast cell growth and differentiation also known as mast cell growth factor. Lesions were graded as I (well differentiated) II (intermediate differentiation) or III (poorly differentiated) according to the following morphologic features: invasiveness, cellularity and cellular morphology, mitotic index and stromal reaction. Immunohistochemical KIT expression was compared with histological grade and some histomorphologic features (cell differentiation and nuclear grade) singularly evaluated. A possible predictive role of biologic behaviour in mast cell tumors for KIT expression was also investigated. Immunohistochemical analysis revealed three different patterns of KIT expression: a cytoplasmic diffuse pattern, a membranous pattern with immunopositivity located in the cell membrane and a cytoplasmic perinuclear pattern where KIT expression was detected in the cytoplasm of the neoplastic mast cells, close to the nucleus. Statistical analysis performed by Pearson method and using the  test has showed a close relationship between different KIT immunohistochemical patterns and histological grade (P=0.00000), cell differentiation (P= 0.00000) and nuclear grade (0.0024). Kaplan & Meier estimated survival curves compared by the survival analysis revealed KIT expression significantly associated to survival time (P= 0.037) but not to cancer free interval (0.50). KIT expression may be considered a useful parameter of malignancy in cutaneous mast cell tumors just like other well known histomorphological features and showed also a predictive role of biologic behaviour of these tumors.
Expression of the KIT protein (CD117) in primary cutaneous mast cell tumors of the dog / PREZIOSI R.; MORINI M.; SARLI G.. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - STAMPA. - 16:(2004), pp. 554-561.
Expression of the KIT protein (CD117) in primary cutaneous mast cell tumors of the dog
PREZIOSI, ROSARIO;MORINI, MARIA;SARLI, GIUSEPPE
2004
Abstract
Thirty-one canine cutaneous nodules, diagnosed as mast cell tumors by histopathologic analysis on hematoxylin and eosin (HE) and toluidine blue stained sections, were used in this study to evaluate the immunohistochemical pattern of expression of KIT protein (CD117), a type III tyrosine kinase protein that is the receptor for stem cell factor (SCF), a cytokine that stimulates mast cell growth and differentiation also known as mast cell growth factor. Lesions were graded as I (well differentiated) II (intermediate differentiation) or III (poorly differentiated) according to the following morphologic features: invasiveness, cellularity and cellular morphology, mitotic index and stromal reaction. Immunohistochemical KIT expression was compared with histological grade and some histomorphologic features (cell differentiation and nuclear grade) singularly evaluated. A possible predictive role of biologic behaviour in mast cell tumors for KIT expression was also investigated. Immunohistochemical analysis revealed three different patterns of KIT expression: a cytoplasmic diffuse pattern, a membranous pattern with immunopositivity located in the cell membrane and a cytoplasmic perinuclear pattern where KIT expression was detected in the cytoplasm of the neoplastic mast cells, close to the nucleus. Statistical analysis performed by Pearson method and using the test has showed a close relationship between different KIT immunohistochemical patterns and histological grade (P=0.00000), cell differentiation (P= 0.00000) and nuclear grade (0.0024). Kaplan & Meier estimated survival curves compared by the survival analysis revealed KIT expression significantly associated to survival time (P= 0.037) but not to cancer free interval (0.50). KIT expression may be considered a useful parameter of malignancy in cutaneous mast cell tumors just like other well known histomorphological features and showed also a predictive role of biologic behaviour of these tumors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
