Bovine chromosome 14 (BTA14) was scanned for QTL affecting milk yield, protein percentage and yield, fat percentage and yield and somatic cell count. Selective milk DNA pooling strategy was applied in a daughter design for eight Holstein-Friesian sires. For each sire-trait combination, milk pools were constructed comprising ~200 best or worst daughters ranked by their EBV or DYD. The sires were genotyped for 16 microsatellites covering BTA14, for the A232K and VNTR mutations at the DGAT1 locus and for a SNP of the tyroglobulin gene. The milk pools were genotyped at the microsatellites heterozygous in their sire. Shadow corrected estimates of allele frequencies were calculated and differences in sire allele frequencies between high and low pools were computed. An adjusted false discovery rate was applied to calculate chromosome-wide significant levels and the data were analysed using an approximate interval mapping procedure. The results indicate the presence of one/more QTL at the centromeric end affecting most traits and one/more putative QTL in the middle-distal part influencing mainly protein percentage.
The BovMAS: Analysis of BTA14 for QTL influencing milk yeld, milk composition and health traits in the Italian Holstein-Friesian cattle breed
FONTANESI, LUCA;SCOTTI, EMILIO;ZAMBONELLI, PAOLO;DALL'OLIO, STEFANIA;BIGI, DANIELE;DAVOLI, ROBERTA;RUSSO, VINCENZO
2005
Abstract
Bovine chromosome 14 (BTA14) was scanned for QTL affecting milk yield, protein percentage and yield, fat percentage and yield and somatic cell count. Selective milk DNA pooling strategy was applied in a daughter design for eight Holstein-Friesian sires. For each sire-trait combination, milk pools were constructed comprising ~200 best or worst daughters ranked by their EBV or DYD. The sires were genotyped for 16 microsatellites covering BTA14, for the A232K and VNTR mutations at the DGAT1 locus and for a SNP of the tyroglobulin gene. The milk pools were genotyped at the microsatellites heterozygous in their sire. Shadow corrected estimates of allele frequencies were calculated and differences in sire allele frequencies between high and low pools were computed. An adjusted false discovery rate was applied to calculate chromosome-wide significant levels and the data were analysed using an approximate interval mapping procedure. The results indicate the presence of one/more QTL at the centromeric end affecting most traits and one/more putative QTL in the middle-distal part influencing mainly protein percentage.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
