Objectives: The aim of the present study was to evaluate the effects of nonthermal extremely high-frequency microwave radiations in a plant-based bioassay, represented by tobacco plants reacting to tobacco mosaic virus with a hypersensitive response leading to the appearance of necrotic lesions at the infection sites. Design: This study was performed blind and different experimental protocols on tobacco plants inoculated with tobacco mosaic virus were used. Bio-objects: Tobacco plants (Nicotiana tabacum L. cultivar Samsun) carrying the resistance gene N against tobacco mosaic virus. Interventions: Tobacco plants or leaf disks were either directly or indirectly (water-mediated) irradiated using a medical device, designed for microwave resonance therapy. It produces nonthermal weak-intensity extremely high-frequency radiations, either modulated at extremely low frequency or in continuous flux of waves, coupled with a nonthermal red/near-infrared radiation. Outcome measurements: The working variable was the number of hypersensitive lesions per leaf disk. Results: Both direct and indirect nonthermal microwave radiations led to significant effects on the hypersensitive response of tobacco plants: modulated radiations generally induced a resistance increase, whereas a continuous flux of waves induced a resistance decrease with direct treatments only. Conclusions: Nonthermal microwave radiations are effective on the hypersensitive response of tobacco to tobacco mosaic virus and their low-frequency modulation seems to be more bioactive than the continuous-flux of waves, particularly in the indirect water-mediated treatments.
Nonthermal Microwave Radiations Affect the Hypersensitive Response of Tobacco to Tobacco Mosaic Virus / L. Betti; G. Trebbi; L. Lazzarato; M. Brizzi; G. L. Calzoni; F. Marinelli; D. Nani; F. Borghini. - In: JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE. - ISSN 1075-5535. - STAMPA. - 10:(2004), pp. 947-957. [10.1089/acm.2004.10.947]
Nonthermal Microwave Radiations Affect the Hypersensitive Response of Tobacco to Tobacco Mosaic Virus
BETTI, LUCIETTA;TREBBI, GRAZIA;LAZZARATO, LISA;BRIZZI, MAURIZIO;CALZONI, GIAN LORENZO;
2004
Abstract
Objectives: The aim of the present study was to evaluate the effects of nonthermal extremely high-frequency microwave radiations in a plant-based bioassay, represented by tobacco plants reacting to tobacco mosaic virus with a hypersensitive response leading to the appearance of necrotic lesions at the infection sites. Design: This study was performed blind and different experimental protocols on tobacco plants inoculated with tobacco mosaic virus were used. Bio-objects: Tobacco plants (Nicotiana tabacum L. cultivar Samsun) carrying the resistance gene N against tobacco mosaic virus. Interventions: Tobacco plants or leaf disks were either directly or indirectly (water-mediated) irradiated using a medical device, designed for microwave resonance therapy. It produces nonthermal weak-intensity extremely high-frequency radiations, either modulated at extremely low frequency or in continuous flux of waves, coupled with a nonthermal red/near-infrared radiation. Outcome measurements: The working variable was the number of hypersensitive lesions per leaf disk. Results: Both direct and indirect nonthermal microwave radiations led to significant effects on the hypersensitive response of tobacco plants: modulated radiations generally induced a resistance increase, whereas a continuous flux of waves induced a resistance decrease with direct treatments only. Conclusions: Nonthermal microwave radiations are effective on the hypersensitive response of tobacco to tobacco mosaic virus and their low-frequency modulation seems to be more bioactive than the continuous-flux of waves, particularly in the indirect water-mediated treatments.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.