The cytochrome P450 catalyses the oxidation of a wide variety of compounds by inserting a single atom of molecular oxygen into the substrate. Concerning to molluscs, the MFO system (or its component) has been identified in different tissues of several bivalve, and the highest activity was found in the digestive gland. The aim of this study is to prove, by immunochemical analysis, the presence and the induction in the bivalve mollusc Scapharca inaequivalvis of two different isoforms of cytochrome P450 (CYP): 1) CYP 1A, that responds specifically to various PAHs and PCBs and 2) CYP 2E which is induced by alcohols, benzene and alkylnitrosamines. Since cytochrome P450 is a key enzyme in the detoxification systems, the induction of specific forms of P450 can be used as a particular biomarker of environmental pollution
IMMUNOCHEMICAL INVESTIGATION OF TWO FORMS OF CYTOCHROME P450 IN SCAPHARCA INAEQUIVALVIS AFTER BENZO[a] PYRENE EXPOSURE
MONARI, MARTA;SERRAZANETTI, GIAN PAOLO;CORTESI, PAOLO;CARPENE', EMILIO;CATTANI, OTELLO
2004
Abstract
The cytochrome P450 catalyses the oxidation of a wide variety of compounds by inserting a single atom of molecular oxygen into the substrate. Concerning to molluscs, the MFO system (or its component) has been identified in different tissues of several bivalve, and the highest activity was found in the digestive gland. The aim of this study is to prove, by immunochemical analysis, the presence and the induction in the bivalve mollusc Scapharca inaequivalvis of two different isoforms of cytochrome P450 (CYP): 1) CYP 1A, that responds specifically to various PAHs and PCBs and 2) CYP 2E which is induced by alcohols, benzene and alkylnitrosamines. Since cytochrome P450 is a key enzyme in the detoxification systems, the induction of specific forms of P450 can be used as a particular biomarker of environmental pollutionI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
