In activation-induced manganese-enhanced MRI (AIM-MRI) experiments, differential accumulation of Mn in activated and silent brain areas is generally assessed using T1-weighted images and quantified by the enhancement of signal intensity (SI), calculated with reference to SI before Mn administration or to SI of brain regions unaffected by the specific stimulus. However, SI enhancement can be unreliable when animals are removed from and reinserted into the magnet. We have developed an experimental protocol based on repeated intraperitoneal (i.p.) injections of Mn, quantitative determination of T1, and coregistration of images to a rat brain atlas that allows absolute quantification of Mn concentration in selected brain areas. Results showed that interanimal variability of postcontrast T1 values was very low (compared to the experimental error in T1 determinations) allowing detection of differential regional Mn uptake in stimulated and unstimulated animals. In addition we have determined in vivo relaxivity of Mn in brain tissue and its frequency dependence.

Experimental protocol for activation-induced manganese-enhanced MRI (AIM-MRI) based on quantitative determination of Mn content in rat brain by fastT1mapping / S. Tambalo;A. Daducci;S. Fiorini;F. Boschi;M. Mariani;M. Marinone;A. Sbarbati;P. Marzola. - In: MAGNETIC RESONANCE IN MEDICINE. - ISSN 0740-3194. - ELETTRONICO. - 62:(2009), pp. 1080-1084. [10.1002/mrm.22095]

Experimental protocol for activation-induced manganese-enhanced MRI (AIM-MRI) based on quantitative determination of Mn content in rat brain by fastT1mapping

MARIANI, MANUEL;
2009

Abstract

In activation-induced manganese-enhanced MRI (AIM-MRI) experiments, differential accumulation of Mn in activated and silent brain areas is generally assessed using T1-weighted images and quantified by the enhancement of signal intensity (SI), calculated with reference to SI before Mn administration or to SI of brain regions unaffected by the specific stimulus. However, SI enhancement can be unreliable when animals are removed from and reinserted into the magnet. We have developed an experimental protocol based on repeated intraperitoneal (i.p.) injections of Mn, quantitative determination of T1, and coregistration of images to a rat brain atlas that allows absolute quantification of Mn concentration in selected brain areas. Results showed that interanimal variability of postcontrast T1 values was very low (compared to the experimental error in T1 determinations) allowing detection of differential regional Mn uptake in stimulated and unstimulated animals. In addition we have determined in vivo relaxivity of Mn in brain tissue and its frequency dependence.
2009
Experimental protocol for activation-induced manganese-enhanced MRI (AIM-MRI) based on quantitative determination of Mn content in rat brain by fastT1mapping / S. Tambalo;A. Daducci;S. Fiorini;F. Boschi;M. Mariani;M. Marinone;A. Sbarbati;P. Marzola. - In: MAGNETIC RESONANCE IN MEDICINE. - ISSN 0740-3194. - ELETTRONICO. - 62:(2009), pp. 1080-1084. [10.1002/mrm.22095]
S. Tambalo;A. Daducci;S. Fiorini;F. Boschi;M. Mariani;M. Marinone;A. Sbarbati;P. Marzola
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/153370
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