BACKGROUND: Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo. METHODS: We compared blood DCs and monocyte-derived DCs from heart-transplant patients undergoing an acute HCMV infection with DCs obtained from HCMV-negative transplant patients. Diagnosis of active HCMV infection was established by antigenemia test. RESULTS: We detected viral RNA and antigens in defined DC subsets obtained from patients undergoing an active HCMV infection. In addition, we found an impaired immunophenotype in immature DCs from HCMV-positive subjects and a reduced ability of mature DCs from the same group of patients to stimulate allogenic T-cell proliferation. CONCLUSIONS: The impaired immunophenotype and function detected in DCs from transplant patients undergoing an active HCMV infection may be a mechanism used by the virus to interfere with early immune functions and thereby contributing to the HCMV-induced immunosuppression in these patients.

Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection / Varani S; Frascaroli G; Gibellini D; Potena L; Lazzarotto T; Lemoli RM; Magelli C; Soderberg-Naucler C; Landini MP.. - In: TRANSPLANTATION. - ISSN 0041-1337. - STAMPA. - 79:(2005), pp. 219-227. [10.1097/01.TP.0000147359.63158.29]

Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection.

VARANI, STEFANIA;FRASCAROLI, GIADA;GIBELLINI, DAVIDE;POTENA, LUCIANO;LAZZAROTTO, TIZIANA;LEMOLI, ROBERTO MASSIMO;MAGELLI, CARLO;LANDINI, MARIA PAOLA
2005

Abstract

BACKGROUND: Human cytomegalovirus (HCMV) infects dendritic cells (DCs) in vitro and inhibits their maturation properties and their ability to stimulate T-cell proliferation and cytotoxicity. This study analyzed HCMV infection of DCs in vivo. METHODS: We compared blood DCs and monocyte-derived DCs from heart-transplant patients undergoing an acute HCMV infection with DCs obtained from HCMV-negative transplant patients. Diagnosis of active HCMV infection was established by antigenemia test. RESULTS: We detected viral RNA and antigens in defined DC subsets obtained from patients undergoing an active HCMV infection. In addition, we found an impaired immunophenotype in immature DCs from HCMV-positive subjects and a reduced ability of mature DCs from the same group of patients to stimulate allogenic T-cell proliferation. CONCLUSIONS: The impaired immunophenotype and function detected in DCs from transplant patients undergoing an active HCMV infection may be a mechanism used by the virus to interfere with early immune functions and thereby contributing to the HCMV-induced immunosuppression in these patients.
2005
Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection / Varani S; Frascaroli G; Gibellini D; Potena L; Lazzarotto T; Lemoli RM; Magelli C; Soderberg-Naucler C; Landini MP.. - In: TRANSPLANTATION. - ISSN 0041-1337. - STAMPA. - 79:(2005), pp. 219-227. [10.1097/01.TP.0000147359.63158.29]
Varani S; Frascaroli G; Gibellini D; Potena L; Lazzarotto T; Lemoli RM; Magelli C; Soderberg-Naucler C; Landini MP.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/14058
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