Plant disease-resistance genes show high conserved regions corresponding to characteristic amino acid domains which make it possible to isolate Resistance Gene Analogs (RGAs) with PCR using degenerate or heterologous primers. Putative RGAs were cloned and sequenced from apricot (Stark Early Orange) and pear (Harrow Sweet, US309 and Old Home). An AFLP-RGA technique was also used in a pear progeny to identify putative disease resistance polymorphic markers.
THE RGA FAMILY. PROMISING GENE ANALOG RELATED TO FIREBLIGHT AND SHARKA RESISTANCE IN PEAR AND APRICOT
TARTARINI, STEFANO;SANSAVINI, SILVIERO
2004
Abstract
Plant disease-resistance genes show high conserved regions corresponding to characteristic amino acid domains which make it possible to isolate Resistance Gene Analogs (RGAs) with PCR using degenerate or heterologous primers. Putative RGAs were cloned and sequenced from apricot (Stark Early Orange) and pear (Harrow Sweet, US309 and Old Home). An AFLP-RGA technique was also used in a pear progeny to identify putative disease resistance polymorphic markers.File in questo prodotto:
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