Bioplastic materials are gaining increasing interest in a variety of different industrial and 13 domestic applications. Beside its usage as mulching films and plant clips in horticulture, no other 14 agricultural applications have been proposed. In 2009 we demonstrated that granules made of the 15 bioplastic Mater-Bi are an efficient and practical solution for field applications of biocontrol 16 isolates of the fungus Aspergillus flavus. Here, we investigated the feasibility to extend this 17 approach for delivering propagules of two biocontrol Trichoderma isolates with the final objective 18 to control damping-off of selected bedding, horticultural and turfgrass species. Bioplastic granules 19 entrapping conidia of Trichoderma virens Gv29-8 or T. harzianum ATCC 20847 were applied to a 20 potting mix infested with the fungal pathogen Rhizoctonia solani. Both isolates showed a positive 21 effect in reducing damping-off disease of impatiens and tomato seedlings. In the case of potting mix 22 infested with the lowest level of pathogen propagules causing 30% of damping-off, application of 23 1% (w/w) of inoculated granules resulted in 5 and 3% of impatiens and tomato seedlings, 24 respectively, affected by damping-off. With a more severe infestation (50% of damping-off), 25 amending the potting mixture with 10% bioplastic granules produced an average of 91% 26 suppression of damping-off in both species. Granules entrapping conidia of the biocontrol isolate 27 ATCC 20847 were significantly more effective in protecting impatiens and tomato seedlings. The 28 experiment was repeated with potting mix infested with Pythium ultimum and planted with annual 29 bluegrass. Bioplastic granules inoculated with the T. harzianum isolate significantly reduced (~ 30 90%) the impact of damping-off caused by this pathogen. Results from this study indicated that the 31 usage of this bioplastic-based formulation has the potential to be extended for the biocontrol of 32 damping-off in flori/horticulture crops and turfgrass.
Extending the Use of Bioplastic Granules for the Application of Trichoderma Biocontrol Isolates in Flori/Horticulture and Turfgrass / Accinelli C.; Mencarelli M.; Abbas H.K.; Waever M.A.. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - STAMPA. - 1015:1015(2014), pp. 1.79-1.88. (Intervento presentato al convegno International CIPA Conference on Plasticulture for a Green Planet tenutosi a Tel Aviv nel 15/05/2012-17/05/2012).
Extending the Use of Bioplastic Granules for the Application of Trichoderma Biocontrol Isolates in Flori/Horticulture and Turfgrass
ACCINELLI, CESARE;MENCARELLI, MARIANGELA;
2014
Abstract
Bioplastic materials are gaining increasing interest in a variety of different industrial and 13 domestic applications. Beside its usage as mulching films and plant clips in horticulture, no other 14 agricultural applications have been proposed. In 2009 we demonstrated that granules made of the 15 bioplastic Mater-Bi are an efficient and practical solution for field applications of biocontrol 16 isolates of the fungus Aspergillus flavus. Here, we investigated the feasibility to extend this 17 approach for delivering propagules of two biocontrol Trichoderma isolates with the final objective 18 to control damping-off of selected bedding, horticultural and turfgrass species. Bioplastic granules 19 entrapping conidia of Trichoderma virens Gv29-8 or T. harzianum ATCC 20847 were applied to a 20 potting mix infested with the fungal pathogen Rhizoctonia solani. Both isolates showed a positive 21 effect in reducing damping-off disease of impatiens and tomato seedlings. In the case of potting mix 22 infested with the lowest level of pathogen propagules causing 30% of damping-off, application of 23 1% (w/w) of inoculated granules resulted in 5 and 3% of impatiens and tomato seedlings, 24 respectively, affected by damping-off. With a more severe infestation (50% of damping-off), 25 amending the potting mixture with 10% bioplastic granules produced an average of 91% 26 suppression of damping-off in both species. Granules entrapping conidia of the biocontrol isolate 27 ATCC 20847 were significantly more effective in protecting impatiens and tomato seedlings. The 28 experiment was repeated with potting mix infested with Pythium ultimum and planted with annual 29 bluegrass. Bioplastic granules inoculated with the T. harzianum isolate significantly reduced (~ 30 90%) the impact of damping-off caused by this pathogen. Results from this study indicated that the 31 usage of this bioplastic-based formulation has the potential to be extended for the biocontrol of 32 damping-off in flori/horticulture crops and turfgrass.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
