This work focuses on the enrichment of a trichloroethylene (TCE) and 1,1,2,2-tetrachloroethane (TeCA) cometabolizing aerobic microbial consortium from a contaminated site in Northern Italy, for the development of an on-site bioremediation treatment. The goals were: (i) to select the best growth substrate and the best-performing consortium in terms of contaminant degradation capability; (ii) to perform a preliminary suspended-cell kinetic test; (iii) to perform a preliminary evaluation of the feasibility of the co-metabolic treatment of the tested groundwater in a packed-bed reactor operating at the site’s temperature (15°C). The best degradation results were obtained with a butane-growing consortium. At 15° C, the selected culture resulted in short lag-times for the onset of butane utilization (< 1 day) and TCE and TeCA degradation (< 1 - 8 days). Once immobilized on 4 different porous carriers, the selected consortium was able to grow and form a stable biofilm. The TCE and TeCA degradation rates resulted similar for the 4 carriers tested. Further experiments are in progress to estimate the kinetic parameters of the co-metabolic processes and to understand which carrier leads to the best performances in a continuous-flow packed-bed reactor process.
Aerobic cometabolic bioremediation of an aquifer polluted by chlorinated solvents: preliminary investigation of the feasibility of a packed bed reactor on-site process / R. Ciavarelli; G. Bucchi; F. Doria; R. Salviulo; G. Zanaroli; S. Fraraccio; D. Frascari; D. Pinelli; F. Fava. - ELETTRONICO. - (2012), pp. 73-76. (Intervento presentato al convegno 5th International Symposium on Biosorption and Bioremediation tenutosi a Prague, Czech Republic nel June 24-28, 2012).
Aerobic cometabolic bioremediation of an aquifer polluted by chlorinated solvents: preliminary investigation of the feasibility of a packed bed reactor on-site process
CIAVARELLI, ROBERTA;BUCCHI, GIACOMO;ZANAROLI, GIULIO;FRARACCIO, SERENA;FRASCARI, DARIO;PINELLI, DAVIDE;FAVA, FABIO
2012
Abstract
This work focuses on the enrichment of a trichloroethylene (TCE) and 1,1,2,2-tetrachloroethane (TeCA) cometabolizing aerobic microbial consortium from a contaminated site in Northern Italy, for the development of an on-site bioremediation treatment. The goals were: (i) to select the best growth substrate and the best-performing consortium in terms of contaminant degradation capability; (ii) to perform a preliminary suspended-cell kinetic test; (iii) to perform a preliminary evaluation of the feasibility of the co-metabolic treatment of the tested groundwater in a packed-bed reactor operating at the site’s temperature (15°C). The best degradation results were obtained with a butane-growing consortium. At 15° C, the selected culture resulted in short lag-times for the onset of butane utilization (< 1 day) and TCE and TeCA degradation (< 1 - 8 days). Once immobilized on 4 different porous carriers, the selected consortium was able to grow and form a stable biofilm. The TCE and TeCA degradation rates resulted similar for the 4 carriers tested. Further experiments are in progress to estimate the kinetic parameters of the co-metabolic processes and to understand which carrier leads to the best performances in a continuous-flow packed-bed reactor process.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.