A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiation
A system biology approach to ionizing radiation response by mammalian cell lines / A. Virelli; E. Gavoci; G. Castellani; I. Zironi; E. Ceccolini; F. Rocchi; M. Sumini; E. Belligotti. - STAMPA. - (2011), pp. 99-104. (Intervento presentato al convegno Sysbiohealth Symposium 2011 tenutosi a Bologna nel 14-15 Dec. 2011).
A system biology approach to ionizing radiation response by mammalian cell lines
VIRELLI, ANGELA;GAVOCI, ENTELE;CASTELLANI, GASTONE;ZIRONI, ISABELLA;CECCOLINI, ELISA;ROCCHI, FEDERICO;SUMINI, MARCO;
2011
Abstract
A key issue when analyzing the biological effects of ionizing radiation is the selection of the biological endpoints. In this study we evaluated the effects of X-rays and protons on membrane ionic currents, cell growth and stress response. The membrane ionic currents amplitude showed a significant nonlinear effects. Growth curves showed a significant dose dependent decrease in the proliferation rate. The fluorescence data suggest a trend in which superoxide intracellular concentration decreases at lower irradiation doses compared to control and higher doses. These results are consistent with a signal transduction pathway where the concentration of superoxide is controlled by type and dose of ionizing radiationI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.