In this paper, we describe a new method to obtain D2O/D2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.

A New Method for D2O/H2O Exchange in Infrared Spectroscopy of Proteins

MALFERRARI, MARCO;VENTUROLI, GIOVANNI;FRANCIA, FRANCESCO;
2012

Abstract

In this paper, we describe a new method to obtain D2O/D2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides. The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.
2012
Malferrari M.; Venturoli G.; Francia F.; Mezzetti A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/124081
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