Ion channels are transmembrane proteins responsible of cell signaling and a large part of pharmaceutical compounds are interacting with them. In-vitro testing of ion channels is a promising technique for high throughput screening (HTS) in drug discovery and personalized medicine. Automated tests of single ion channels embedded in artificial bilayer lipid membranes (BLM) is gaining attention over patch clamp technique due to its characteristic of performing parallel tests on selected sets of channels or multiple pharmaceutical targets. However, BLM arrays formation is a critical process based on manual and time-consuming techniques. In this paper, an automatic liquid dispensing system for BLM formation monitored in real-time by using low-noise current amplifiers is presented. As proof of this approach, concurrent formation of BLMs is shown. Additionally, single ion channel recordings on an automatically formed BLM is presented and discussed.

A Parallel Sensing Technique for Automatic Bilayer Lipid Membrane Arrays Monitoring

ROSSI, MICHELE;THEI, FEDERICO;TARTAGNI, MARCO
2012

Abstract

Ion channels are transmembrane proteins responsible of cell signaling and a large part of pharmaceutical compounds are interacting with them. In-vitro testing of ion channels is a promising technique for high throughput screening (HTS) in drug discovery and personalized medicine. Automated tests of single ion channels embedded in artificial bilayer lipid membranes (BLM) is gaining attention over patch clamp technique due to its characteristic of performing parallel tests on selected sets of channels or multiple pharmaceutical targets. However, BLM arrays formation is a critical process based on manual and time-consuming techniques. In this paper, an automatic liquid dispensing system for BLM formation monitored in real-time by using low-noise current amplifiers is presented. As proof of this approach, concurrent formation of BLMs is shown. Additionally, single ion channel recordings on an automatically formed BLM is presented and discussed.
SENSORS & TRANSDUCERS
M. Rossi; F. Thei; M. Tartagni
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/122810
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