Stallions are not selected for fertility, and breeders can demand the use of frozen semen of high valuable stallions despite a poor quality after thawing.The aim of this study was to verify if density gradient centrifugation of low quality frozenthawed semencanimprove sperm quality and fertility rates after deep horn uterine insemination. Low quality frozen-thawed semen, recovered from one stallion with a breeding report of no pregnancy, was centrifuged on a discontinuous density gradient. Sperm recovery rate, motility and membrane integrity were assessed.Four mares were inseminated for two consecutive cycles with low dose-low volume deep horn technique. Sperm quality and viability was improved after gradient centrifugation, but any of the mares became pregnant after insemination.
Density gradient centrifugation of frozen-thawed equine semen with low motility and fertility / Mari G; Mislei B; Rizzato G; Dardi M; Iacono E; Merlo B. - ELETTRONICO. - (2012), pp. 55-57. (Intervento presentato al convegno 10th Congress of Italian Society of Animal Reproduction (SIRA 2012)1st Balkan Conference on Biology and Reproduction in Farm Animals and in AquacultureTirana, 23-25 May, 2012 tenutosi a Tirana nel 23-25 Maggio 2012) [10.4488/2012.SIRA.C.001].
Density gradient centrifugation of frozen-thawed equine semen with low motility and fertility.
MARI, GAETANO;MISLEI, BEATRICE;RIZZATO, GIOVANNI;IACONO, ELEONORA;MERLO, BARBARA
2012
Abstract
Stallions are not selected for fertility, and breeders can demand the use of frozen semen of high valuable stallions despite a poor quality after thawing.The aim of this study was to verify if density gradient centrifugation of low quality frozenthawed semencanimprove sperm quality and fertility rates after deep horn uterine insemination. Low quality frozen-thawed semen, recovered from one stallion with a breeding report of no pregnancy, was centrifuged on a discontinuous density gradient. Sperm recovery rate, motility and membrane integrity were assessed.Four mares were inseminated for two consecutive cycles with low dose-low volume deep horn technique. Sperm quality and viability was improved after gradient centrifugation, but any of the mares became pregnant after insemination.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
