Standard Biological Parts (pTet-LacI-pLac-LacY-GFP) from the iGEM Registry were used to design a synthetic circuit operated by IPTG as an extracellular stimulus to trigger gene transcription. The circuit was designed to work as a Schmitt Trigger (switch with hysteresis). A genetic program without the LacY gene (open-loop configuration) was first cloned into a high copy number plasmid and was used in E. coli to identify a mathematical model of the molecular circuit. Then, the model was used to predict the behavior in closed-loop and to assess the existence of a switch with hysteresis.

A Synthetic Genetic Circuit to Implement a Schmitt Trigger in E.coli

CERONI, FRANCESCA;PASINI, ALICE;GIORDANO, EMANUELE DOMENICO;
2008

Abstract

Standard Biological Parts (pTet-LacI-pLac-LacY-GFP) from the iGEM Registry were used to design a synthetic circuit operated by IPTG as an extracellular stimulus to trigger gene transcription. The circuit was designed to work as a Schmitt Trigger (switch with hysteresis). A genetic program without the LacY gene (open-loop configuration) was first cloned into a high copy number plasmid and was used in E. coli to identify a mathematical model of the molecular circuit. Then, the model was used to predict the behavior in closed-loop and to assess the existence of a switch with hysteresis.
2008
Book of Abstract “IET Conference on Synthetic Biology, Systems Biology and Bioinformatics” (BioSysBio 2008)
100
101
CERONI F.; PASINI A.; GIORDANO E.; CAVALCANTI S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/114145
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