Sex-sorting process induces several changes in sperm cell functionality which are defined as capacitation-like modifications. The aim of the present study was to evaluate protein tyrosine phosphorylation (TP) status and Chlortetracycline (CTC) staining pattern in boar spermatozoa after sex-sorting procedure and to compare these parameters with those of freshly ejaculated, capacitated and acrosome reacted cells. TP status was evaluated by immunofluorescence and three different patterns were recognized; each of them was considered to be typical of fresh (F), capacitated (C) and acrosome reacted(AR) cells. In sorted spermatozoa the most expressed pattern was F (80.2 ± 6.6% Mean ± SD), while C and AR patterns were 8.5 ± 5.9% and 11.3 ± 5.6%, respectively. These pattern distribution is similar to that observed in freshly ejaculated sperm cells (F 87.5 ± 7.3%; C 9.9 ± 6.1%; AR 2.6 ± 2%). CTC positivity was assayed in that it is considered a sensitive capacitation index. Sex sorted cells presented the following pattern distribution: F 68.4 ± 5.2%; C 26.3 ± 4.3%; AR 5.3 ± 0.9%, which are very similar to the those observed in capacitated spermatozoa (F 67.8 ± 6.1%; C 25.2 ± 2.5%; AR 7 ± 4.3%). These results suggest that sex sorting procedure induces a capacitation-like switch in sperm subpopulations of boar ejaculates, as registered with CTC technique. As for protein TP immunoreactivity, it evidences a fresh-like subpopulation trend, with an increase of AR pattern, probably due to mechanical damage. Further studies would be necessary to better define the pathways involved in sex sortinginduced modifications.
Capacitation-like changes in sex-sorted boar spermatozoa / Diego Bucci; Juan Enrique Rodriguez-Gil; Claudia Vallorani; Carlo Tamanini; Giovanna Galeati; Marcella Spinaci. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - STAMPA. - 46 suppl. 3:(2011), pp. 91-91. (Intervento presentato al convegno The 15th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR). tenutosi a Antalya, Turkey nel 15-17 September 2011).
Capacitation-like changes in sex-sorted boar spermatozoa
BUCCI, DIEGO;VALLORANI, CLAUDIA;TAMANINI, CARLO;GALEATI, GIOVANNA;SPINACI, MARCELLA
2011
Abstract
Sex-sorting process induces several changes in sperm cell functionality which are defined as capacitation-like modifications. The aim of the present study was to evaluate protein tyrosine phosphorylation (TP) status and Chlortetracycline (CTC) staining pattern in boar spermatozoa after sex-sorting procedure and to compare these parameters with those of freshly ejaculated, capacitated and acrosome reacted cells. TP status was evaluated by immunofluorescence and three different patterns were recognized; each of them was considered to be typical of fresh (F), capacitated (C) and acrosome reacted(AR) cells. In sorted spermatozoa the most expressed pattern was F (80.2 ± 6.6% Mean ± SD), while C and AR patterns were 8.5 ± 5.9% and 11.3 ± 5.6%, respectively. These pattern distribution is similar to that observed in freshly ejaculated sperm cells (F 87.5 ± 7.3%; C 9.9 ± 6.1%; AR 2.6 ± 2%). CTC positivity was assayed in that it is considered a sensitive capacitation index. Sex sorted cells presented the following pattern distribution: F 68.4 ± 5.2%; C 26.3 ± 4.3%; AR 5.3 ± 0.9%, which are very similar to the those observed in capacitated spermatozoa (F 67.8 ± 6.1%; C 25.2 ± 2.5%; AR 7 ± 4.3%). These results suggest that sex sorting procedure induces a capacitation-like switch in sperm subpopulations of boar ejaculates, as registered with CTC technique. As for protein TP immunoreactivity, it evidences a fresh-like subpopulation trend, with an increase of AR pattern, probably due to mechanical damage. Further studies would be necessary to better define the pathways involved in sex sortinginduced modifications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
