Inverted open microwells for analysis and functional sorting of single live cells

We present a novel method for the isolation, functional analysis and sorting of single cells or small cellular aggregates. In- verted open microwells featuring a microchannel on top, an open air-fluid interface on bottom side and embedded electrodes were implemented on flexible-PCB technology. Microwell arrays in pitch with standard microtiter plates were implemented. K562 cells were delivered to the microwells and trapped at the air-flow interface. Cell delivery and aggregate formation were controlled by dielectrophoresis. Continuous fluid replacement around the cells during the experiment was demonstrated. After analysis, live cells were easily recovered to standard plates where cell growth was demonstrated.