This study was focused on the effects of virus and phytoplasma infections on the production of Echinacea purpurea (L.) Moench secondary metabolites, such as caffeic acid derivatives, alkamides and essential oil. The identification of caffeic acid derivatives and alkamides was carried out by means of HPLC-DAD, HPLC-ESI-MS and MS2. Quantitative analysis of these compounds was carried out using HPLC-DAD. The results indicated that the presence of the two pathogens significantly decrease (P < 0.05) the content of cichoric acid, the main caffeic acid derivative. Regarding the main alkamide, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide, a significant decrease (P < 0.05) in the content of this secondary metabolite was observed in virus-infected plants in comparison with healthy plants, while in the phytoplasma-infected sample the variation of this secondary metabolite was not appreciable. The % relative area of the E/Z isomers of this alkamide was also found to change in infected samples. The GC and GC-MS analysis of E. purpurea essential oil enabled the identification of thirty compounds. The main significant differences (P < 0.05) in the semi-quantitative composition were observed for three components: limonene, cis-verbenol and verbenone. The results indicate that the presence of virus and phytoplasma has an appreciable influence on the content of E. purpurea secondary metabolites, which is an important issue in defining commercial quality, market value and therapeutic efficacy of this herbal drug.
Chromatographic Methods for Metabolite Profiling of Virus- and Phytoplasma-infected Plants of Echinacea purpurea / F.Pellati; F.Epifano; N.Contaldo; G.Orlandini; L.Cavicchi; S.Genovese; D.Bertelli; S.Benvenuti; M.Curini; A.Bertaccini; M.G.Bellardi. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - STAMPA. - 59:(2011), pp. 10425-10434. [10.1021/jf2025677]
Chromatographic Methods for Metabolite Profiling of Virus- and Phytoplasma-infected Plants of Echinacea purpurea
CONTALDO, NICOLETTA;CAVICCHI, LISA;BERTACCINI, ASSUNTA;BELLARDI, MARIA GRAZIA
2011
Abstract
This study was focused on the effects of virus and phytoplasma infections on the production of Echinacea purpurea (L.) Moench secondary metabolites, such as caffeic acid derivatives, alkamides and essential oil. The identification of caffeic acid derivatives and alkamides was carried out by means of HPLC-DAD, HPLC-ESI-MS and MS2. Quantitative analysis of these compounds was carried out using HPLC-DAD. The results indicated that the presence of the two pathogens significantly decrease (P < 0.05) the content of cichoric acid, the main caffeic acid derivative. Regarding the main alkamide, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide, a significant decrease (P < 0.05) in the content of this secondary metabolite was observed in virus-infected plants in comparison with healthy plants, while in the phytoplasma-infected sample the variation of this secondary metabolite was not appreciable. The % relative area of the E/Z isomers of this alkamide was also found to change in infected samples. The GC and GC-MS analysis of E. purpurea essential oil enabled the identification of thirty compounds. The main significant differences (P < 0.05) in the semi-quantitative composition were observed for three components: limonene, cis-verbenol and verbenone. The results indicate that the presence of virus and phytoplasma has an appreciable influence on the content of E. purpurea secondary metabolites, which is an important issue in defining commercial quality, market value and therapeutic efficacy of this herbal drug.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
